Exercise training restores IGFIR survival signaling in d-galactose induced-aging rats to suppress cardiac apoptosis.
Aging
Cardiac apoptosis
Cell survival
Fibrosis
Senescence
Journal
Journal of advanced research
ISSN: 2090-1232
Titre abrégé: J Adv Res
Pays: Egypt
ID NLM: 101546952
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
16
03
2020
revised:
26
05
2020
accepted:
17
06
2020
entrez:
28
12
2020
pubmed:
29
12
2020
medline:
29
12
2020
Statut:
epublish
Résumé
Insulin-like growth factor-I receptor (IGF1R) mediated survival signaling is a crucial mechanism for cellular endurance and a potential indicator of recuperation in deteriorating hearts. This study evaluates the impact of long-term exercise training in enhancing cardiac survival mechanism in D-galactose-induced toxicity associated aging rats. Forty-eight male SD-rats were segregated into 4 groups (n=9) and were named as control, exercise training groups, aging group and aging group with exercise training. Aging was induced by intraperitoneal (IP) D-galactose (150 mL/kg) injection for 8 weeks and for exercise training, the rats were left to swim in warm water for 60 min every day and 5 times/week. Western blotting of proteins from the left ventricles was performed to identify the modulations in the survival signaling. Tissue sections were analyzed to determine the extent of fibrosis and apoptosis. Western-blot analysis performed on the excised left ventricles (LV) showed that proteins of the cardiac survival pathway including IGF1R and Akt and the pro-survival Bcl-2 showed significant decrease in the aging group, whereas the levels were restored in the aging rats subjected to exercise training. In addition, aging groups showed increased interstitial space and collagen accumulation. Further, TUNEL assay showed higher number of apoptotic cells in the LV of aging group, which was correlated with increase in the proteins involved in FAS-FADD-dependent apoptosis. However, these aging associated effects were ameliorated upon exercise training in the D-galactose-induced aging rats that showed elevated IGF1R/Akt signaling. The results suggest that IGFIR survival signaling cascadeis elevated in following long-term exercise training and thereby provide cardio-protective benefits in D-galactose induced aging rats.
Identifiants
pubmed: 33364043
doi: 10.1016/j.jare.2020.06.015
pii: S2090-1232(20)30124-7
pmc: PMC7753223
doi:
Types de publication
Journal Article
Langues
eng
Pagination
35-41Informations de copyright
© 2020 The Authors. Published by Elsevier B.V. on behalf of Cairo University.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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