Combinatorial nanodot stripe assay to systematically study cell haptotaxis.
Nanofabrication and nanopatterning
Nanoscale materials
Journal
Microsystems & nanoengineering
ISSN: 2055-7434
Titre abrégé: Microsyst Nanoeng
Pays: England
ID NLM: 101695458
Informations de publication
Date de publication:
2020
2020
Historique:
received:
16
07
2020
revised:
16
09
2020
accepted:
13
10
2020
entrez:
28
12
2020
pubmed:
29
12
2020
medline:
29
12
2020
Statut:
epublish
Résumé
Haptotaxis is critical to cell guidance and development and has been studied in vitro using either gradients or stripe assays that present a binary choice between full and zero coverage of a protein cue. However, stripes offer only a choice between extremes, while for gradients, cell receptor saturation, migration history, and directional persistence confound the interpretation of cellular responses. Here, we introduce nanodot stripe assays (NSAs) formed by adjacent stripes of nanodot arrays with different surface coverage. Twenty-one pairwise combinations were designed using 0, 1, 3, 10, 30, 44 and 100% stripes and were patterned with 200 × 200, 400 × 400 or 800 × 800 nm
Identifiants
pubmed: 33365138
doi: 10.1038/s41378-020-00223-0
pii: 223
pmc: PMC7735170
doi:
Types de publication
Journal Article
Langues
eng
Pagination
114Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Conflict of interestThe authors declare that they have no conflict of interest.
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