PROSS 2: a new server for the design of stable and highly expressed protein variants.
Journal
Bioinformatics (Oxford, England)
ISSN: 1367-4811
Titre abrégé: Bioinformatics
Pays: England
ID NLM: 9808944
Informations de publication
Date de publication:
09 Apr 2021
09 Apr 2021
Historique:
received:
05
08
2020
revised:
10
12
2020
accepted:
15
12
2020
medline:
29
12
2020
pubmed:
29
12
2020
entrez:
28
12
2020
Statut:
ppublish
Résumé
Many natural and designed proteins are only marginally stable limiting their usefulness in research and applications. Recently, we described an automated structure and sequence-based design method, called PROSS, for optimizing protein stability and heterologous expression levels that has since been validated on dozens of proteins. Here, we introduce improvements to the method, workflow and presentation, including more accurate sequence analysis, error handling and automated analysis of the quality of the sequence alignment that is used in design calculations. PROSS2 is freely available for academic use at https://pross.weizmann.ac.il.
Identifiants
pubmed: 33367682
pii: 6050711
doi: 10.1093/bioinformatics/btaa1071
pmc: PMC7611707
mid: EMS109272
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
123-125Subventions
Organisme : European Research Council
ID : 815379
Pays : International
Organisme : European Research Council
ID : 815379
Pays : International
Organisme : Israel Science Foundation
ID : 1844
Organisme : Israel Ministry of Science
ID : 128625
Organisme : Milner Foundation
Informations de copyright
© The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.