Automation of Continuous-Rotation Data Collection for MicroED.
3D
CryoEM
Electron crystallography
Electron diffraction
Electron microscopy
MicroED
Microcrystal
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
28
12
2020
pubmed:
29
12
2020
medline:
26
3
2021
Statut:
ppublish
Résumé
Automated coordination of microscope and camera functions for MicroED data collection simplifies the procedure for robust dataset acquisition and enables unattended sequential collection of many crystal targets. This chapter discusses the prerequisites for an algorithm of data collection automation for continuous-rotation MicroED and presents a practical protocol for achieving this goal using the popular TEM control software program SerialEM.
Identifiants
pubmed: 33368012
doi: 10.1007/978-1-0716-0966-8_16
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
321-327Subventions
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Références
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