Quantitative detection of SARS-CoV-2 RNA in nasopharyngeal samples from infected patients with mild disease.
Adolescent
Adult
Aged
Aged, 80 and over
COVID-19
/ diagnosis
COVID-19 Testing
/ methods
Child
Child, Preschool
Diagnostic Tests, Routine
Female
Humans
Infant
Male
Middle Aged
Nasopharynx
/ virology
Polyproteins
/ genetics
RNA, Viral
/ isolation & purification
Real-Time Polymerase Chain Reaction
/ methods
Ribonuclease P
/ genetics
SARS-CoV-2
/ genetics
Viral Load
Viral Proteins
/ genetics
Young Adult
SARS-CoV-2
nasopharyngeal samples
quantitative detection of SARS-CoV-2 RNA
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
03
09
2020
revised:
08
12
2020
accepted:
22
12
2020
pubmed:
29
12
2020
medline:
20
3
2021
entrez:
28
12
2020
Statut:
ppublish
Résumé
Diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) cases is based on the count of real-time reverse transcription-plymerase chain reaction (RT-PCR) positive people. Viral load by real-time RT-PCR has been suggested as a biomarker of the SARS-CoV-2 infection. However, the association of viral load and severity of the disease is not yet resolved. Nasopharyngeal samples from 458 patients were tested by RT-PCR for SARS-CoV-2 diagnosis. Relative quantitation was made by the comparative threshold cycle (ΔΔC
Substances chimiques
ORF1ab polyprotein, SARS-CoV-2
0
Polyproteins
0
RNA, Viral
0
Viral Proteins
0
Ribonuclease P
EC 3.1.26.5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2439-2445Informations de copyright
© 2020 Wiley Periodicals LLC.
Références
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