Chrysin protects against testicular toxicity caused by lead acetate in rats with its antioxidant, anti-inflammatory, and antiapoptotic properties.
Caspase-3
NF-κB
chrysin
lead acetate
oxidative damage
testicular toxicity
Journal
Journal of food biochemistry
ISSN: 1745-4514
Titre abrégé: J Food Biochem
Pays: United States
ID NLM: 7706045
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
21
09
2020
revised:
10
11
2020
accepted:
04
12
2020
pubmed:
29
12
2020
medline:
9
7
2021
entrez:
28
12
2020
Statut:
ppublish
Résumé
In the present study, the protective effects of chrysin (CHR) against testicular damage caused by lead acetate (PbAc) were examined. In this way, 30 min after rats were given 25 and 50 mg/kg/b.w CHR orally for seven consecutive days, 30 mg/kg/b.w PbAc was administered orally. In biochemical analysis of testicular tissue, it was found that PbAc-reduced antioxidant parameters [glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT)], while it increased lipid peroxidation, inflammatory markers [nuclear factor kappa-B (NF-κB), cyclooxygenase-2 (COX-2), prostaglandin E2 (PGE-2), and inducible nitric oxide synthase (iNOS)], and 8-hydroxy-2'-deoxyguanosine (8-OHdG). In the immunohistochemical examination, it was determined that PbAc increased the expression of tumor necrosis factor-α (TNF-α) and caspase-3. Accordingly, PbAc was found to cause a decrease in sperm motility and an increase in the percentage of dead sperm. However, it has been observed that CHR relieves oxidative stress due to its antioxidant properties, thus protecting against inflammation and apoptosis. It also allowed the CHR sperm parameters to return to control group levels. The results revealed that CHR could be a natural substance to be used in Pb-induced testicular toxicity. PRACTICAL APPLICATIONS: Lead (Pb) is an important environmental contaminant heavy metal. Pb is believed to reduce fertility in men. Oxidative stress plays a significant role in the damage caused by Pb to testicular tissue. CHR is an antioxidant substance that occurs naturally in various plants and has various pharmacological properties. In the present study, it was investigated whether CHR has a protective effect against testicular toxicity induced by PbAc. The results revealed that in rats, CHR protects the testicular tissue from PbAc toxicity by showing antioxidant, anti-inflammatory and anti-apoptotic effects, thus bringing sperm parameters closer to normal.
Substances chimiques
Anti-Inflammatory Agents
0
Antioxidants
0
Flavonoids
0
Organometallic Compounds
0
chrysin
3CN01F5ZJ5
lead acetate
RX077P88RY
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13593Informations de copyright
© 2020 Wiley Periodicals LLC.
Références
Abdrabou, M. I., Elleithy, E. M. M., Yasin, N. A. E., Shaheen, Y. M., & Galal, M. (2019). Ameliorative effects of Spirulina maxima and Allium sativum on lead acetate-induced testicular injury in male albino rats with respect to caspase-3 gene expression. Acta Histochemica, 121(2), 198-206. https://doi.org/10.1016/j.acthis.2018.12.006
Aebi, H. (1984). Catalase in vitro. In L. Packer (Ed.), Methods in enzymology (Vol. 105, pp. 121-126). San Diego, CA: Elsevier.
Aksu, E. H., Kandemir, F. M., Küçükler, S., & Mahamadu, A. (2018). Improvement in colistin-induced reproductive damage, apoptosis, and autophagy in testes via reducing oxidative stress by chrysin. Journal of Biochemical and Molecular Toxicology, 32(11), e22201. https://doi.org/10.1002/jbt.22201
Aksu, E. H., Ozkaraca, M., Kandemir, F. M., Omur, A. D., Eldutar, E., Kucukler, S., & Comakli, S. (2016). Mitigation of paracetamol-induced reproductive damage by chrysin in male rats via reducing oxidative stress. Andrologia, 48(10), 1145-1154. https://doi.org/10.1111/and.12553
Alcaraz-Contreras, Y., Mendoza-Lozano, R. P., Martinez-Alcaraz, E. R., Martinez-Alfaro, M., Gallegos-Corona, M. A., Ramirez-Morales, M. A., & Vazquez-Guevara, M. A. (2016). Silymarin and dimercaptosuccinic acid ameliorate lead-induced nephrotoxicity and genotoxicity in rats. Human and Experimental Toxicology, 35(4), 398-403. https://doi.org/10.1177/0960327115591373
AL-Megrin, W. A., Alomar, S.Alkhuriji, A. F., Metwally, D. M., Mohamed, S. K., Kassab, R. B., Abdel Moneim, A. E., & El-Khadragy, M. F. (2020). Luteolin protects against testicular injury induced by lead acetate by activating the Nrf2/HO-1 pathway. IUBMB Life.
AL-Megrin, W. A., El-Khadragy, M. F., Hussein, M. H., Mahgoub, S., Abdel-Mohsen, D. M., Taha, H., Bakkar, A. A., Abdel Moneim, A. E., & Amin, H. K. (2020). Green Coffea arabica extract ameliorates testicular injury in high-fat diet/streptozotocin-induced diabetes in rats. Journal of Diabetes Research, 2020, 1-13.
Alves, M. G., Dias, T. R., Silva, B. M., & Oliveira, P. F. (2014). Metabolic cooperation in testis as a pharmacological target: From disease to contraception. Current Molecular Pharmacology, 7(2), 83-95.
Andersen, O., & Aaseth, J. (2016). A review of pitfalls and progress in chelation treatment of metal poisonings. Journal of Trace Elements in Medicine and Biology, 38, 74-80. https://doi.org/10.1016/j.jtemb.2016.03.013
Anjum, M. R., Sainath, S. B., Suneetha, Y., & Reddy, P. S. (2011). Lead acetate induced reproductive and paternal mediated developmental toxicity in rats. Ecotoxicology and Environmental Safety, 74(4), 793-799. https://doi.org/10.1016/j.ecoenv.2010.10.044
Badary, D. M. (2017). Folic acid protects against lead acetate-induced hepatotoxicity by decreasing NF-κB, IL-1β production and lipid peroxidation mediataed cell injury. Pathophysiology, 24(1), 39-44. https://doi.org/10.1016/j.pathophys.2017.02.002
Banerjee, A., Yang, W., Karplus, M., & Verdine, G. L. (2005). Structure of a repair enzyme interrogating undamaged DNA elucidates recognition of damaged DNA. Nature, 434(7033), 612. https://doi.org/10.1038/nature03458
Benzer, F., Kandemir, F. M., Kucukler, S., Comakli, S., & Caglayan, C. (2018). Chemoprotective effects of curcumin on doxorubicin-induced nephrotoxicity in wistar rats: By modulating inflammatory cytokines, apoptosis, oxidative stress and oxidative DNA damage. Archives of Physiology and Biochemistry, 124(5), 448-457. https://doi.org/10.1080/13813455.2017.1422766
Benzer, F., Kandemir, F. M., Ozkaraca, M., Kucukler, S., & Caglayan, C. (2018). Curcumin ameliorates doxorubicin-induced cardiotoxicity by abrogation of inflammation, apoptosis, oxidative DNA damage, and protein oxidation in rats. Journal of Biochemical and Molecular Toxicology, 32(2). https://doi.org/10.1002/jbt.22030
Caglayan, C., Temel, Y., Kandemir, F. M., Yildirim, S., & Kucukler, S. (2018). Naringin protects against cyclophosphamide-induced hepatotoxicity and nephrotoxicity through modulation of oxidative stress, inflammation, apoptosis, autophagy, and DNA damage. Environmental Science and Pollution Research International, 25(21), 20968-20984. https://doi.org/10.1007/s11356-018-2242-5
Celik, H., Kandemir, F. M., Caglayan, C., Ozdemir, S., Comakli, S., Kucukler, S., & Yardim, A. (2020). Neuroprotective effect of rutin against colistin-induced oxidative stress, inflammation and apoptosis in rat brain associated with the CREB/BDNF expressions. Molecular Biology Reports, 47(3), 2023-2034. https://doi.org/10.1007/s11033-020-05302-z
Çelik, H., Kucukler, S., Çomaklı, S., Caglayan, C., Özdemir, S., Yardım, A., Karaman, M., & Kandemir, F. M. (2020). Neuroprotective effect of chrysin on isoniazid-induced neurotoxicity via suppression of oxidative stress, inflammation and apoptosis in rats. Neurotoxicology, 81, 197-208. https://doi.org/10.1016/j.neuro.2020.10.009
Celik, H., Kucukler, S., Comakli, S., Ozdemir, S., Caglayan, C., Yardim, A., & Kandemir, F. M. (2019). Morin attenuates ifosfamide-induced neurotoxicity in rats via suppression of oxidative stress, neuroinflammation and neuronal apoptosis. Neurotoxicology, 76, 126-137. https://doi.org/10.1016/j.neuro.2019.11.004
Celik, H., Kucukler, S., Ozdemir, S., Comakli, S., Gur, C., Kandemir, F. M., & Yardim, A. (2020). Lycopene protects against central and peripheral neuropathy by inhibiting oxaliplatin-induced ATF-6 pathway, apoptosis, inflammation and oxidative stress in brains and sciatic tissues of rats. Neurotoxicology, 80, 29-40. https://doi.org/10.1016/j.neuro.2020.06.005
Del Fabbro, L., Jesse, C. R., de Gomes, M. G., Borges Filho, C., Donato, F., Souza, L. C., Goes, A. R., Furian, A. F., & Boeira, S. P. (2019). The flavonoid chrysin protects against zearalenone induced reproductive toxicity in male mice. Toxicon, 165, 13-21. https://doi.org/10.1016/j.toxicon.2019.04.004
Dorostghoal, M., Seyyednejad, S., & Jabari, A. (2014). Protective effects of Fumaria parviflora L. on lead-induced testicular toxicity in male rats. Andrologia, 46(4), 437-446.
Eldutar, E., Kandemir, F. M., Kucukler, S., & Caglayan, C. (2017). Restorative effects of Chrysin pretreatment on oxidant-antioxidant status, inflammatory cytokine production, and apoptotic and autophagic markers in acute paracetamol-induced hepatotoxicity in rats: An experimental and biochemical study. Journal of Biochemical and Molecular Toxicology, 31(11), e21960. https://doi.org/10.1002/jbt.21960
Elgawish, R. A. R., & Abdelrazek, H. M. (2014). Effects of lead acetate on testicular function and caspase-3 expression with respect to the protective effect of cinnamon in albino rats. Toxicology Reports, 1, 795-801. https://doi.org/10.1016/j.toxrep.2014.10.010
El-khadragy, M., Al-Megrin, W. A., AlSadhan, N. A., Metwally, D. M., El-Hennamy, R. E., Salem, F. E. H., & Abdel Moneim, A. E. (2020). Impact of coenzyme Q10 administration on lead acetate-induced testicular damage in rats. Oxidative medicine and cellular longevity, 2020, 1-12.
El-Sayed, Y. S., & El-Neweshy, M. S. (2010). Impact of lead toxicity on male rat reproduction at “hormonal and histopathological levels”. Toxicological and Environ Chemistry, 92(4), 765-774. https://doi.org/10.1080/02772240902984453
Ezejiofor, A. N., & Orisakwe, O. E. (2019). The protective effect of Costus afer Ker Gawl aqueous leaf extract on lead-induced reproductive changes in male albino Wistar rats. JBRA Assisted Reproduction, 23(3), 215.
Feng, C., Liu, S., Zhou, F., Gao, Y., Li, Y., Du, G., Chen, Y., Jiao, H., Feng, J., Zhang, Y., Bo, D., Li, Z., & Fan, G. (2019). Oxidative stress in the neurodegenerative brain following lifetime exposure to lead in rats: Changes in lifespan profiles. Toxicology, 411, 101-109. https://doi.org/10.1016/j.tox.2018.11.003
Gandhi, J., Hernandez, R. J., Chen, A., Smith, N. L., Sheynkin, Y. R., Joshi, G., & Khan, S. A. (2017). Impaired hypothalamic-pituitary-testicular axis activity, spermatogenesis, and sperm function promote infertility in males with lead poisoning. Zygote, 25(2), 103-110. https://doi.org/10.1017/S0967199417000028
Hanedan, B., Ozkaraca, M., Kirbas, A., Kandemir, F. M., Aktas, M. S., Kilic, K., Comakli, S., Kucukler, S., & Bilgili, A. (2018). Investigation of the effects of hesperidin and chrysin on renal injury induced by colistin in rats. Biomedicine & Pharmacotherapy, 108, 1607-1616. https://doi.org/10.1016/j.biopha.2018.10.001
Hassan, E., Kahilo, K., Kamal, T., El-Neweshy, M., & Hassan, M. (2019). Protective effect of diallyl sulfide against lead-mediated oxidative damage, apoptosis and down-regulation of CYP19 gene expression in rat testes. Life Sciences, 226, 193-201. https://doi.org/10.1016/j.lfs.2019.04.020
Hassan, E., Kahilo, K., Kamal, T., Hassan, M., & Saleh Elgawish, M. (2019). The protective effect of epigallocatechin-3-gallate on testicular oxidative stress in lead-induced toxicity mediated by Cyp19 gene / estradiol level. Toxicology, 422, 76-83. https://doi.org/10.1016/j.tox.2019.04.015
Hernandez-Ochoa, I., Garcia-Vargas, G., Lopez-Carrillo, L., Rubio-Andrade, M., Moran-Martinez, J., Cebrian, M. E., & Quintanilla-Vega, B. (2005). Low lead environmental exposure alters semen quality and sperm chromatin condensation in northern Mexico. Reproductive Toxicology, 20(2), 221-228. https://doi.org/10.1016/j.reprotox.2005.01.007
Jana, K., Samanta, P. K., & De, D. K. (2010). Nicotine diminishes testicular gametogenesis, steroidogenesis, and steroidogenic acute regulatory protein expression in adult albino rats: Possible influence on pituitary gonadotropins and alteration of testicular antioxidant status. Toxicological Sciences, 116(2), 647-659. https://doi.org/10.1093/toxsci/kfq149
Jomova, K., & Valko, M. (2011). Advances in metal-induced oxidative stress and human disease. Toxicology, 283(2-3), 65-87. https://doi.org/10.1016/j.tox.2011.03.001
Kandemir, F., Benzer, E., Ozkaraca, M., Ceribasi, S., Yildirim, N. C., & Ozdemir, N. (2012). Protective antioxidant effects of grape seed extract in a cisplatin-induced hepatotoxicity model in rabbits. Revue de Médecine Vétérinaire, 163(11), 539-545.
Kandemir, F. M., Caglayan, C., Aksu, E. H., Yildirim, S., Kucukler, S., Gur, C., & Eser, G. (2020). Protective effect of rutin on mercuric chloride-induced reproductive damage in male rats. Andrologia, 52(3), e13524. https://doi.org/10.1111/and.13524
Kandemir, F. M., Küçükler, S., & Çağlayan, C. (2017). Beneficial effects of silymarin and naringin against methotrexate-induced hepatotoxicity in rats. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 12(2), 167-177.
Kandemir, F. M., Kucukler, S., Caglayan, C., Gur, C., Batil, A. A., & Gülçin, İ. (2017). Therapeutic effects of silymarin and naringin on methotrexate-induced nephrotoxicity in rats: Biochemical evaluation of anti-inflammatory, antiapoptotic, and antiautophagic properties. Journal of Food Biochemistry, 41(5), e12398. https://doi.org/10.1111/jfbc.12398
Ko, E. Y., Sabanegh, E. S. Jr, & Agarwal, A. (2014). Male infertility testing: Reactive oxygen species and antioxidant capacity. Fertility and Sterility, 102(6), 1518-1527. https://doi.org/10.1016/j.fertnstert.2014.10.020
Kucukler, S., Benzer, F., Yildirim, S., Gur, C., Kandemir, F. M., Bengu, A. S., Ayna, A., Caglayan, C., & Dortbudak, M. B. (2020). Protective effects of chrysin against oxidative stress and inflammation induced by lead acetate in rat kidneys: A biochemical and histopathological approach. Biological Trace Element Research. https://doi.org/10.1007/s12011-020-02268-8
Kucukler, S., Caglayan, C., Darendelioglu, E., & Kandemir, F. M. (2020). Morin attenuates acrylamide-induced testicular toxicity in rats by regulating the NF-kappaB, Bax/Bcl-2 and PI3K/Akt/mTOR signaling pathways. Life Sciences, 261, 118301. https://doi.org/10.1016/j.lfs.2020.118301
Kucukler, S., Darendelioglu, E., Caglayan, C., Ayna, A., Yildirim, S., & Kandemir, F. M. (2020). Zingerone attenuates vancomycin-induced hepatotoxicity in rats through regulation of oxidative stress, inflammation and apoptosis. Life Sciences, 259, 118382. https://doi.org/10.1016/j.lfs.2020.118382
Lapidot, T., Walker, M. D., & Kanner, J. (2002). Antioxidant and prooxidant effects of phenolics on pancreatic beta-cells in vitro. Journal of Agriculture and Food Chemistry, 50(25), 7220-7225. https://doi.org/10.1021/jf020615a
Lawrence, R. A., & Burk, R. F. (1976). Glutathione peroxidase activity in selenium-deficient rat liver. Biochemical and Biophysical Research Communications, 71(4), 952-958. https://doi.org/10.1016/0006-291X(76)90747-6
Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193(1), 265-275.
Mancuso, F., Arato, I., Lilli, C., Bellucci, C., Bodo, M., Calvitti, M., Aglietti, M. C., dell'Omo, M., Nastruzzi, C., Calafiore, R., Luca, G., & Marinucci, L. (2018). Acute effects of lead on porcine neonatal Sertoli cells in vitro. Toxicology in Vitro, 48, 45-52. https://doi.org/10.1016/j.tiv.2017.12.013
Metwally, D. M., Alajmi, R. A., El-Khadragy, M. F., Yehia, H. M., AL-Megrin, W. A., Akabawy, A. M. A., Amin, H. K., & Abdel Moneim, A. E. (2020). Chlorogenic acid confers robust neuroprotection against arsenite toxicity in mice by reversing oxidative stress, inflammation, and apoptosis. Journal of Functional Foods, 75, 104202. https://doi.org/10.1016/j.jff.2020.104202
Naz, S., Imran, M., Rauf, A., Orhan, I. E., Shariati, M. A., Shahbaz, M., Qaisrani, T. B., Shah, Z. A., Plygun, S., & Heydari, M. (2019). Chrysin: Pharmacological and therapeutic properties. Life Sciences, 235, 116797.
Oeckinghaus, A., & Ghosh, S. (2009). The NF-κB family of transcription factors and its regulation. Cold Spring Harbor Perspectives in Biology, 1(4), a000034.
Omobowale, T. O., Oyagbemi, A. A., Akinrinde, A. S., Saba, A. B., Daramola, O. T., Ogunpolu, B. S., & Olopade, J. O. (2014). Failure of recovery from lead induced hepatoxicity and disruption of erythrocyte antioxidant defence system in Wistar rats. Environmental Toxicology and Pharmacology, 37(3), 1202-1211. https://doi.org/10.1016/j.etap.2014.03.002
Placer, Z. A., Cushman, L. L., & Johnson, B. C. (1966). Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Analytical Biochemistry, 16(2), 359-364. https://doi.org/10.1016/0003-2697(66)90167-9
Rani, N., Bharti, S., Bhatia, J., Nag, T., Ray, R., & Arya, D. S. (2016). Chrysin, a PPAR-γ agonist improves myocardial injury in diabetic rats through inhibiting AGE-RAGE mediated oxidative stress and inflammation. Chemico-biological Interactions, 250, 59-67. https://doi.org/10.1016/j.cbi.2016.03.015
Rao, F., Zhai, Y., & Sun, F. (2016). Punicalagin mollifies lead acetate-induced oxidative imbalance in male reproductive system. International Journal of Molecular Sciences, 17(8), 1269. https://doi.org/10.3390/ijms17081269
Richburg, J. H. (2000). The relevance of spontaneous-and chemically-induced alterations in testicular germ cell apoptosis to toxicology. Toxicology Letters, 112, 79-86. https://doi.org/10.1016/S0378-4274(99)00253-2
Sainath, S., Meena, R., Supriya, C., Reddy, K. P., & Reddy, P. S. (2011). Protective role of Centella asiatica on lead-induced oxidative stress and suppressed reproductive health in male rats. Environmental Toxicology and Pharmacology, 32(2), 146-154.
Sun, Y., Oberley, L. W., & Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clinical Chemistry, 34(3), 497-500. https://doi.org/10.1093/clinchem/34.3.497
Türk, G., Ateşşahin, A., Sönmez, M., Çeribaşi, A. O., & Yüce, A. (2008). Improvement of cisplatin-induced injuries to sperm quality, the oxidant-antioxidant system, and the histologic structure of the rat testis by ellagic acid. Fertility and Sterility, 89(5), 1474-1481. https://doi.org/10.1016/j.fertnstert.2007.04.059
Vaseem, H., Singh, V. K., & Singh, M. P. (2017). Heavy metal pollution due to coal washery effluent and its decontamination using a macrofungus, Pleurotus ostreatus. Ecotoxicology and Environmental Safety, 145, 42-49. https://doi.org/10.1016/j.ecoenv.2017.07.001
Wang, H., Zhai, N., Chen, Y., Xu, H., & Huang, K. (2017). Cadmium induces Ca2+ mediated, calpain-1/caspase-3-dependent apoptosis in primary cultured rat proximal tubular cells. Journal of Inorganic Biochemistry, 172, 16-22. https://doi.org/10.1016/j.jinorgbio.2017.04.005
Wang, J., Yang, Z., Lin, L., Zhao, Z., Liu, Z., & Liu, X. (2012). Protective effect of naringenin against lead-induced oxidative stress in rats. Biological Trace Element Research, 146(3), 354-359. https://doi.org/10.1007/s12011-011-9268-6
Wani, A. L., Ara, A., & Usmani, J. A. (2015). Lead toxicity: A review. Interdisciplinary Toxicology, 8(2), 55-64. https://doi.org/10.1515/intox-2015-0009
Xia, D., Yu, X., Liao, S., Shao, Q., Mou, H., & Ma, W. (2010). Protective effect of Smilax glabra extract against lead-induced oxidative stress in rats. Journal of Ethnopharmacology, 130(2), 414-420. https://doi.org/10.1016/j.jep.2010.05.025
Yardim, A., Kandemir, F. M., Ozdemir, S., Kucukler, S., Comakli, S., Gur, C., & Celik, H. (2020). Quercetin provides protection against the peripheral nerve damage caused by vincristine in rats by suppressing caspase 3, NF-κB, ATF-6 pathways and activating Nrf2, Akt pathways. Neurotoxicology. https://doi.org/10.1016/j.neuro.2020.10.001
Yardım, A., Kucukler, S., Özdemir, S., Çomaklı, S., Caglayan, C., Kandemir, F. M., & Çelik, H. (2020). Silymarin alleviates docetaxel-induced central and peripheral neurotoxicity by reducing oxidative stress, inflammation and apoptosis in rats. Gene, 145239. https://doi.org/10.1016/j.gene.2020.145239