Breast Cancer Organoids Model Patient-Specific Response to Drug Treatment.
YAP
breast cancer
dasatinib
drug testing
heterogeneity
mechanotransduction
patient-derived tumor organoids
statin
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
21 Dec 2020
21 Dec 2020
Historique:
received:
15
10
2020
revised:
15
12
2020
accepted:
17
12
2020
entrez:
29
12
2020
pubmed:
30
12
2020
medline:
30
12
2020
Statut:
epublish
Résumé
Tumor organoids are tridimensional cell culture systems that are generated in vitro from surgically resected patients' tumors. They can be propagated in culture maintaining several features of the tumor of origin, including cellular and genetic heterogeneity, thus representing a promising tool for precision cancer medicine. Here, we established patient-derived tumor organoids (PDOs) from different breast cancer subtypes (luminal A, luminal B, human epidermal growth factor receptor 2 (HER2)-enriched, and triple negative). The established model systems showed histological and genomic concordance with parental tumors. However, in PDOs, the ratio of diverse cell populations was frequently different from that originally observed in parental tumors. We showed that tumor organoids represent a valuable system to test the efficacy of standard therapeutic treatments and to identify drug resistant populations within tumors. We also report that inhibitors of mechanosignaling and of Yes-associated protein 1 (YAP) activation can restore chemosensitivity in drug resistant tumor organoids.
Identifiants
pubmed: 33371412
pii: cancers12123869
doi: 10.3390/cancers12123869
pmc: PMC7770601
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : European Union, European Regional Development Fund and Interreg V-A Italia-Austria 2014-2020
ID : ITAT1096-P
Organisme : Italian University and Research Ministry
ID : PRIN-2017HWTP2K_004
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 22174
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 22759
Organisme : Associazione Italiana per la Ricerca sul Cancro
ID : 10016
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