Invasion of Mycoplasma bovis into bovine synovial cells utilizing the clathrin-dependent endocytosis pathway.
Cell invasion
Endocytosis
Mycoplasma bovis
Mycoplasmaarthritis
Synovial cell
Journal
Veterinary microbiology
ISSN: 1873-2542
Titre abrégé: Vet Microbiol
Pays: Netherlands
ID NLM: 7705469
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
27
10
2020
accepted:
08
12
2020
pubmed:
30
12
2020
medline:
6
8
2021
entrez:
29
12
2020
Statut:
ppublish
Résumé
Mycoplasma bovis causes chronic arthritis in cattle, accompanied by a severe inflammatory reaction of the joints. Recent studies demonstrated that M. bovis can invade bovine non-phagocytic cells, but the mechanism of M. bovis internalization in the cells remains unclear. In this study, we examined the mechanism by which M. bovis invades synovial cells, including the pathway of cell invasion. Using fluorescence and electron microscopy, multiple M. bovis were observed to adhere to and be internalized in cultured bovine synovial cells. The number of M. bovis colocalized with clathrin heavy chain (CLTC) per cell was significantly higher than the number of M. bovis colocalized with caveolin-1 (Cav-1). The internalized ratio of M. bovis in synovial cells treated with clathrin-dependent endocytosis inhibitor and small interfering RNA (siRNA) against CLTC was significantly lower than that in control cells. In contrast, the internalized ratio of M. bovis in synovial cells was unaffected by siRNA against Cav-1. These findings provide the first evidence that clathrin-dependent endocytosis is one of the major pathways by which M. bovis invades into synovial cells.
Identifiants
pubmed: 33373880
pii: S0378-1135(20)31094-4
doi: 10.1016/j.vetmic.2020.108956
pii:
doi:
Substances chimiques
Adhesins, Bacterial
0
Clathrin
0
RNA, Small Interfering
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108956Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.