Simultaneous Quantification of Antioxidants Paraxanthine and Caffeine in Human Saliva by Electrochemical Sensing for CYP1A2 Phenotyping.
CYP1A2 phenotyping
antioxidants
caffeine
differential pulse voltammetry
human saliva
paraxanthine
Journal
Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
Titre abrégé: Antioxidants (Basel)
Pays: Switzerland
ID NLM: 101668981
Informations de publication
Date de publication:
24 Dec 2020
24 Dec 2020
Historique:
received:
19
11
2020
revised:
15
12
2020
accepted:
21
12
2020
entrez:
30
12
2020
pubmed:
31
12
2020
medline:
31
12
2020
Statut:
epublish
Résumé
The enzyme CYP1A2 is responsible for the metabolism of numerous antioxidants in the body, including caffeine, which is transformed into paraxanthine, its main primary metabolite. Both molecules are known for their antioxidant and pro-oxidant characteristics, and the paraxanthine-to-caffeine molar ratio is a widely accepted metric for CYP1A2 phenotyping, to optimize dose-response effects in individual patients. We developed a simple, cheap and fast electrochemical based method for the simultaneous quantification of paraxanthine and caffeine in human saliva, by differential pulse voltammetry, using an anodically pretreated glassy carbon electrode. Cyclic voltammetry experiments revealed for the first time that the oxidation of paraxanthine is diffusion controlled with an irreversible peak at ca. +1.24 V (vs. Ag/AgCl) in a 0.1 M H
Identifiants
pubmed: 33374269
pii: antiox10010010
doi: 10.3390/antiox10010010
pmc: PMC7823619
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : Grant Agreement no. 642014 (IPCOS).
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