Use of biocompatible redox-active polymers based on carbon nanotubes and modified organic matrices for development of a highly sensitive BOD biosensor.

Biochemical oxygen demand Bovine serum albumin Electron transfer mediators Paracoccus yeei bacteria Redox-active polymers Single-walled carbon nanotubes

Journal

Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761

Informations de publication

Date de publication:
Feb 2021
Historique:
received: 15 07 2020
revised: 26 10 2020
accepted: 12 11 2020
entrez: 30 12 2020
pubmed: 31 12 2020
medline: 19 8 2021
Statut: ppublish

Résumé

This work investigated the use of redox-active polymers based on bovine serum albumin and chitosan, covalently bound to mediators neutral red and ferrocene and containing carbon nanotubes, for immobilization of Paracoccus yeei VKM B-3302 bacteria. The structures of produced polymers were studied by IR spectroscopy and scanning electron microscopy. Cyclic voltammetry and impedance spectroscopy found the electrochemical characteristics of the investigated systems: the heterogeneous electron transfer rate constant, the constant of the rate of interaction with P. yeei bacteria and the impedance. The systems containing carbon nanotubes and ferrocene-based redox-active polymer proved to be the most promising. Biosensors formed using the hybrid polymers had a high sensitivity with the lower boundary of 0.1 mg/dm

Identifiants

pubmed: 33375974
pii: S0141-0229(20)30199-X
doi: 10.1016/j.enzmictec.2020.109706
pii:
doi:

Substances chimiques

Nanotubes, Carbon 0
Polymers 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

109706

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

V A Arlyapov (VA)

Tula State University, 92 Lenin Pr., Tula, 300012, Russia.

A S Kharkova (AS)

Tula State University, 92 Lenin Pr., Tula, 300012, Russia.

S K Kurbanaliyeva (SK)

Tula State University, 92 Lenin Pr., Tula, 300012, Russia.

L S Kuznetsova (LS)

Tula State University, 92 Lenin Pr., Tula, 300012, Russia.

A V Machulin (AV)

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, prosp. Nauki 5, Pushchino, Moscow Region, 142290, Russia.

S E Tarasov (SE)

G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, prosp. Nauki 5, Pushchino, Moscow Region, 142290, Russia.

P V Melnikov (PV)

MIREA - Russian Technological University, 78 Vernadsky Pr., Moscow, 119454, Russia.

O N Ponamoreva (ON)

Tula State University, 92 Lenin Pr., Tula, 300012, Russia.

V A Alferov (VA)

Tula State University, 92 Lenin Pr., Tula, 300012, Russia.

A N Reshetilov (AN)

Tula State University, 92 Lenin Pr., Tula, 300012, Russia; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, prosp. Nauki 5, Pushchino, Moscow Region, 142290, Russia. Electronic address: anatol@ibpm.pushchino.ru.

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Classifications MeSH