Crosstalk between phosphorylation and ubiquitination is involved in high salt-induced WNK4 expression.
WNK4
high salt
hypertension
phosphorylation
ubiquitination
Journal
Experimental and therapeutic medicine
ISSN: 1792-0981
Titre abrégé: Exp Ther Med
Pays: Greece
ID NLM: 101531947
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
24
07
2020
accepted:
24
11
2020
entrez:
30
12
2020
pubmed:
31
12
2020
medline:
31
12
2020
Statut:
ppublish
Résumé
With no lysine 4 (WNK4) is a serine/threonine kinase, which is expressed in the kidney and associated with salt-sensitive hypertension. However, how salt regulates WNK4 remains unclear. In the present study, the C57BL/6 mice and HEK293 cells were treated with high salt and the expression of WNK4 protein and its ubiquitination and phosphorylation levels were detected. Western blotting demonstrated that WNK4 expression was significantly increased in high salt-treated mice and cells. Meanwhile, co-immunoprecipitation analysis demonstrated that the ubiquitination of WNK4 was decreased under high-salt simulation. It was also identified that the Lys-1023 site was the most important ubiquitination site for WNK4, and it was found that phosphorylation at the Ser-1022 site was a prerequisite for ubiquitination. These results suggested that there was crosstalk between phosphorylation and ubiquitination in the WNK4 protein, and high salt may downregulate its phosphorylation and, in turn, decrease its ubiquitination, leading to a decrease in WNK4 degradation. This eventually resulted in an increase in the abundance of WNK4 protein.
Identifiants
pubmed: 33376515
doi: 10.3892/etm.2020.9565
pii: ETM-0-0-09565
pmc: PMC7751476
doi:
Types de publication
Journal Article
Langues
eng
Pagination
133Informations de copyright
Copyright © 2020, Spandidos Publications.
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