Isolation, Serotyping, and Molecular Detection of Bovine FMD Virus from Outbreak Cases in Aba'ala District of Afar Region, Ethiopia.
Journal
Veterinary medicine international
ISSN: 2090-8113
Titre abrégé: Vet Med Int
Pays: United States
ID NLM: 101524203
Informations de publication
Date de publication:
2020
2020
Historique:
received:
30
09
2020
revised:
28
11
2020
accepted:
28
11
2020
entrez:
30
12
2020
pubmed:
31
12
2020
medline:
31
12
2020
Statut:
epublish
Résumé
On the basis of FMDV outbreak cases, a cross-sectional study was undertaken to collect samples from January 2019 to March 2020 intended for isolation, serotyping, and molecular detection of FMDV in the study district. The purposive sampling method was applied to select the study area for the reason of the presence of FMD outbreak case report during the study period. Totally, 27 FMD clinical samples were collected from affected study population during field outbreak. Out of 27 samples, 18 of them were inoculated on cultured Baby hamster kidney (BHK-21) monolayer cells, and all 27 samples were tested using conventional RT-PCR and sets of specific universal primers. Finally, the PCR products were visualized with UV illumination and imaged with gel documentation system. The current study results revealed that out of 18 clinical samples subjected to virus isolation, 72.2% ( Our study finding indicated that FMDV is prevalent in the study area and FMDV serotype SAT-2 was the causality for the outbreaks of the disease in the study area. Hence, region-wise FMD outbreak investigation, further phylogenetic analysis, and vaccine matching field isolates should be carried out for effective vaccine development to control the disease.
Sections du résumé
BACKGROUND
BACKGROUND
On the basis of FMDV outbreak cases, a cross-sectional study was undertaken to collect samples from January 2019 to March 2020 intended for isolation, serotyping, and molecular detection of FMDV in the study district. The purposive sampling method was applied to select the study area for the reason of the presence of FMD outbreak case report during the study period. Totally, 27 FMD clinical samples were collected from affected study population during field outbreak. Out of 27 samples, 18 of them were inoculated on cultured Baby hamster kidney (BHK-21) monolayer cells, and all 27 samples were tested using conventional RT-PCR and sets of specific universal primers. Finally, the PCR products were visualized with UV illumination and imaged with gel documentation system.
RESULTS
RESULTS
The current study results revealed that out of 18 clinical samples subjected to virus isolation, 72.2% (
CONCLUSIONS
CONCLUSIONS
Our study finding indicated that FMDV is prevalent in the study area and FMDV serotype SAT-2 was the causality for the outbreaks of the disease in the study area. Hence, region-wise FMD outbreak investigation, further phylogenetic analysis, and vaccine matching field isolates should be carried out for effective vaccine development to control the disease.
Identifiants
pubmed: 33376591
doi: 10.1155/2020/8847728
pmc: PMC7744179
doi:
Types de publication
Journal Article
Langues
eng
Pagination
8847728Informations de copyright
Copyright © 2020 Teshager Dubie and Tsedale Amare.
Déclaration de conflit d'intérêts
The authors declare that they have no conflicts of interest regarding the publication of this paper.
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