Cell-free DNA (cfDNA) and Exosome Profiling from a Year-Long Human Spaceflight Reveals Circulating Biomarkers.
Omics
Space Medicine
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
18 Dec 2020
18 Dec 2020
Historique:
received:
24
08
2020
revised:
12
10
2020
accepted:
18
11
2020
entrez:
30
12
2020
pubmed:
31
12
2020
medline:
31
12
2020
Statut:
epublish
Résumé
Liquid biopsies based on cell-free DNA (cfDNA) or exosomes provide a noninvasive approach to monitor human health and disease but have not been utilized for astronauts. Here, we profile cfDNA characteristics, including fragment size, cellular deconvolution, and nucleosome positioning, in an astronaut during a year-long mission on the International Space Station, compared to his identical twin on Earth and healthy donors. We observed a significant increase in the proportion of cell-free mitochondrial DNA (cf-mtDNA) inflight, and analysis of post-flight exosomes in plasma revealed a 30-fold increase in circulating exosomes and patient-specific protein cargo (including brain-derived peptides) after the year-long mission. This longitudinal analysis of astronaut cfDNA during spaceflight and the exosome profiles highlights their utility for astronaut health monitoring, as well as cf-mtDNA levels as a potential biomarker for physiological stress or immune system responses related to microgravity, radiation exposure, and the other unique environmental conditions of spaceflight.
Identifiants
pubmed: 33376973
doi: 10.1016/j.isci.2020.101844
pii: S2589-0042(20)31041-5
pmc: PMC7756145
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101844Subventions
Organisme : NIMH NIH HHS
ID : R01 MH117406
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI125416
Pays : United States
Organisme : NIEHS NIH HHS
ID : R01 ES021006
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI129851
Pays : United States
Organisme : NCATS NIH HHS
ID : KL2 TR002385
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS076465
Pays : United States
Organisme : NIBIB NIH HHS
ID : R25 EB020393
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI151059
Pays : United States
Informations de copyright
© 2020 The Author(s).
Déclaration de conflit d'intérêts
S.M.B. is a cofounder and Scientific Advisory Board member of KromaTiD, Inc. C.E.M. is a cofounder and board member for Biotia, Inc. and Onegevity Health, Inc., as well as an advisor or grantee for Abbvie, Inc., ArcBio, Daiichi Sankyo, DNA Genotek, Karius, Inc., and Whole Biome, Inc. D.B. is a cofounder of Poppy Health, Inc. and Analogs Llc.
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