Isolation of Human CD49f
Astrocytes
/ cytology
Biological Assay
/ methods
Cell Differentiation
/ drug effects
Cell Separation
Cells, Cultured
Coculture Techniques
Culture Media, Conditioned
/ pharmacology
Flow Cytometry
Humans
Induced Pluripotent Stem Cells
/ drug effects
Integrin alpha6
/ metabolism
Neurons
/ cytology
Neurotoxins
/ toxicity
Toxicity Tests
Cell Biology
Neuroscience
Stem Cells
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 12 2020
18 12 2020
Historique:
entrez:
30
12
2020
pubmed:
31
12
2020
medline:
31
12
2020
Statut:
epublish
Résumé
Given the critical roles of astrocytes in neuroinflammation and neurological diseases, models for studying human astrocyte biology are in increasing demand. Here, we present a protocol to isolate human astrocytes from induced pluripotent stem cell (iPSC)-based cultures, neural organoids, and primary tissue, using the surface marker CD49f. Moreover, we provide protocols for
Identifiants
pubmed: 33377066
doi: 10.1016/j.xpro.2020.100172
pii: S2666-1667(20)30159-3
pmc: PMC7757411
doi:
Substances chimiques
Culture Media, Conditioned
0
Integrin alpha6
0
Neurotoxins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100172Subventions
Organisme : NINDS NIH HHS
ID : R21 NS111186
Pays : United States
Informations de copyright
© 2020 The Author(s).
Déclaration de conflit d'intérêts
NYSCF. Patent pending.
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