Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs.
Animals
Blood Cells
/ cytology
Cell Culture Techniques
/ methods
Cellular Reprogramming
Green Fluorescent Proteins
/ metabolism
Humans
Induced Pluripotent Stem Cells
/ cytology
Menstruation
/ physiology
Mice
Oocytes
/ metabolism
Stromal Cells
/ metabolism
Transcription Factors
/ metabolism
Virion
/ metabolism
Cell Differentiation
Cell culture
Stem Cells
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 12 2020
18 12 2020
Historique:
entrez:
30
12
2020
pubmed:
31
12
2020
medline:
31
12
2020
Statut:
epublish
Résumé
Cell reprogramming has revolutionized the fields of cell and regenerative biology. However, human induced pluripotent stem cell (iPSC) derivation remains inefficient and variable. Here, we present a protocol that uses human menstrual blood-derived stromal cells (MnSCs), which are susceptible to reprogramming, as a source of somatic cells. We describe an oocyte-based reprogramming combination to generate AOX15-iPSCs that can be used to study different states of pluripotency. For complete details on the use and execution of this protocol, please refer to Lopez-Caraballo et al. (2020).
Identifiants
pubmed: 33377077
doi: 10.1016/j.xpro.2020.100183
pii: S2666-1667(20)30170-2
pmc: PMC7757400
doi:
Substances chimiques
Transcription Factors
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100183Informations de copyright
© 2020 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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