A protocol for displaying viral envelope glycoproteins on the surface of vesicular stomatitis viruses.
Cell culture
Cell-based assays
Immunology
Molecular biology
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
18 12 2020
18 12 2020
Historique:
entrez:
30
12
2020
pubmed:
31
12
2020
medline:
31
12
2020
Statut:
epublish
Résumé
We describe the production of single-cycle (sc) and replication-competent recombinant vesicular stomatitis viruses (rcVSVs) displaying heterologous envelope glycoproteins (Envs) on their surface. We prepare scVSVs by transiently expressing HIV-1 Envs or SARS-CoV-2 spike followed by infection of the cells with scVSV particles, which do not carry the
Identifiants
pubmed: 33377103
doi: 10.1016/j.xpro.2020.100209
pii: S2666-1667(20)30196-9
pmc: PMC7757661
doi:
Substances chimiques
Recombinant Proteins
0
Spike Glycoprotein, Coronavirus
0
env Gene Products, Human Immunodeficiency Virus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100209Subventions
Organisme : NIDA NIH HHS
ID : DP2 DA049279
Pays : United States
Informations de copyright
© 2020 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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