Microscopy-based assay for semi-quantitative detection of SARS-CoV-2 specific antibodies in human sera: A semi-quantitative, high throughput, microscopy-based assay expands existing approaches to measure SARS-CoV-2 specific antibody levels in human sera.
Antibodies, Viral
/ blood
COVID-19
/ diagnosis
COVID-19 Testing
/ methods
Fluorescent Antibody Technique
High-Throughput Screening Assays
Humans
Image Processing, Computer-Assisted
/ statistics & numerical data
Immune Sera
/ chemistry
Immunoassay
Immunoglobulin A
/ blood
Immunoglobulin G
/ blood
Immunoglobulin M
/ blood
Machine Learning
Microscopy
/ methods
SARS-CoV-2
/ immunology
Sensitivity and Specificity
SARS-CoV-2
antibody
immunofluorescence
machine learning image analysis
quantitative microscopy
serological test
Journal
BioEssays : news and reviews in molecular, cellular and developmental biology
ISSN: 1521-1878
Titre abrégé: Bioessays
Pays: United States
ID NLM: 8510851
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
21
09
2020
revised:
24
11
2020
accepted:
30
11
2020
pubmed:
31
12
2020
medline:
9
3
2021
entrez:
30
12
2020
Statut:
ppublish
Résumé
Emergence of the novel pathogenic coronavirus SARS-CoV-2 and its rapid pandemic spread presents challenges that demand immediate attention. Here, we describe the development of a semi-quantitative high-content microscopy-based assay for detection of three major classes (IgG, IgA, and IgM) of SARS-CoV-2 specific antibodies in human samples. The possibility to detect antibodies against the entire viral proteome together with a robust semi-automated image analysis workflow resulted in specific, sensitive and unbiased assay that complements the portfolio of SARS-CoV-2 serological assays. Sensitive, specific and quantitative serological assays are urgently needed for a better understanding of humoral immune response against the virus as a basis for developing public health strategies to control viral spread. The procedure described here has been used for clinical studies and provides a general framework for the application of quantitative high-throughput microscopy to rapidly develop serological assays for emerging virus infections.
Identifiants
pubmed: 33377226
doi: 10.1002/bies.202000257
pmc: PMC7883048
doi:
Substances chimiques
Antibodies, Viral
0
Immune Sera
0
Immunoglobulin A
0
Immunoglobulin G
0
Immunoglobulin M
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000257Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : 240245660
Organisme : Deutsche Forschungsgemeinschaft
ID : 41607209
Organisme : Heisenberg program
Informations de copyright
© 2020 The Authors. BioEssays published by Wiley Periodicals LLC.
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