Hyaluronan/Poly-L-lysine/Berberine Nanogels for Impaired Wound Healing.
berberine
chronic wounds
hyaluronan
nanogels
ε-polylysine
Journal
Pharmaceutics
ISSN: 1999-4923
Titre abrégé: Pharmaceutics
Pays: Switzerland
ID NLM: 101534003
Informations de publication
Date de publication:
28 Dec 2020
28 Dec 2020
Historique:
received:
31
10
2020
revised:
15
12
2020
accepted:
22
12
2020
entrez:
31
12
2020
pubmed:
1
1
2021
medline:
1
1
2021
Statut:
epublish
Résumé
Physiological wound healing process can be delayed in the presence of certain pathologies, such as diabetes or cancer. In this perspective, the aim of this study was to design a new nanogel platform of hyaluronan, poly-L-lysine and berberine suitable for wound treatment. Two different nanogel formulations were selected after a first formulation screening. They were prepared by adding dropwise 2 mg/mL hyaluronan aqueous solution (200 or 700 kDa) to 1.25 mg/mL poly-L-lysine aqueous solution. Blank nanogels formulated with 200 kDa HA resulted stable after freeze-drying with dimensions, polydispersity index and zeta potential of 263.6 ± 13.1 nm, 0.323 ± 0.029 and 32.7 ± 3.5 mV, respectively. Both blank and berberine-loaded nanogels showed rounded-shape structures. Loaded nanogels released nearly 50% of loaded berberine within 45 min, whereas the remaining 50% was released up to 24 h in vitro. Both, blank and berberine-loaded nanogels were able to completely close the fibroblasts gap in 42 h.
Identifiants
pubmed: 33379303
pii: pharmaceutics13010034
doi: 10.3390/pharmaceutics13010034
pmc: PMC7823568
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : PRIN 2017 #20173ZECCM
Organisme : Ministerio de Economía, Industria y Competitividad, Gobierno de España
ID : SAF2017-83118-R
Organisme : Xunta de Galicia
ID : ED431C 2020/17
Organisme : Research Funding for University of Catania
ID : Piano per la Ricerca 2016-2018
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