Looking for genetic effects of polluted anthropized environments on Caiman crocodilus crocodilus (Reptilia, Crocodylia): A comparative genotoxic and chromosomal analysis.
Comet assay
Fluorescence in situ hybridization
Interstitial telomeric sites
Micronucleus test
Nucleolus organizer region
Journal
Ecotoxicology and environmental safety
ISSN: 1090-2414
Titre abrégé: Ecotoxicol Environ Saf
Pays: Netherlands
ID NLM: 7805381
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
08
09
2020
revised:
17
12
2020
accepted:
19
12
2020
pubmed:
1
1
2021
medline:
30
1
2021
entrez:
31
12
2020
Statut:
ppublish
Résumé
The Amazon aquatic ecosystems have been modified by the human population growth, going through changes in their water bodies and aquatic biota. The spectacled alligator (Caiman crocodilus crocodilus) has a wide distribution and adaptability to several environments, even those polluted ones. This study aimed to investigate if a Caiman species living in urban streams of Manaus city (Amazonas State, Brazil) is affected by environmental pollution. For that, it was used classical and molecular cytogenetic procedures, in addition to micronucleus and comet assays. Although the karyotype macrostructure remains unaltered (2 n = 42 chromosomes; 24 t + 18 m/sm; NF = 60), the genotoxic analysis and the cytogenetic mapping of repetitive DNA sequences demonstrated that polluted environments alter the genome of the specimens, affecting both the chromosomal organization and the genetic material.
Identifiants
pubmed: 33383344
pii: S0147-6513(20)31671-7
doi: 10.1016/j.ecoenv.2020.111835
pii:
doi:
Substances chimiques
Mutagens
0
Water Pollutants, Chemical
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111835Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.