O-acetylation controls the glycosylation of bacterial serine-rich repeat glycoproteins.
Acetylation
Amino Acid Sequence
/ genetics
Glycoproteins
/ chemistry
Glycosylation
Glycosyltransferases
/ chemistry
Humans
Membrane Proteins
/ chemistry
Protein Binding
/ genetics
Protein Processing, Post-Translational
/ genetics
Protein Transport
/ genetics
Serine
/ chemistry
Streptococcus gordonii
/ chemistry
O-acetylation
Streptococcus gordonii
accessory
glycoprotein:glucosylation
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
Historique:
received:
19
09
2020
revised:
21
12
2020
accepted:
31
12
2020
pubmed:
2
1
2021
medline:
25
8
2021
entrez:
1
1
2021
Statut:
ppublish
Résumé
The serine-rich repeat (SRR) glycoproteins of gram-positive bacteria are a family of adhesins that bind to a wide range of host ligands, and expression of SRR glycoproteins is linked with enhanced bacterial virulence. The biogenesis of these surface glycoproteins involves their intracellular glycosylation and export via the accessory Sec system. Although all accessory Sec components are required for SRR glycoprotein export, Asp2 of Streptococcus gordonii also functions as an O-acetyltransferase that modifies GlcNAc residues on the SRR adhesin gordonii surface protein B (GspB). Because these GlcNAc residues can also be modified by the glycosyltransferases Nss and Gly, it has been unclear whether the post-translational modification of GspB is coordinated. We now report that acetylation modulates the glycosylation of exported GspB. Loss of O-acetylation due to aps2 mutagenesis led to the export of GspB glycoforms with increased glucosylation of the GlcNAc moieties. Linkage analysis of the GspB glycan revealed that both O-acetylation and glucosylation occurred at the same C6 position on GlcNAc residues and that O-acetylation prevented Glc deposition. Whereas streptococci expressing nonacetylated GspB with increased glucosylation were significantly reduced in their ability to bind human platelets in vitro, deletion of the glycosyltransferases nss and gly in the asp2 mutant restored platelet binding to WT levels. These findings demonstrate that GlcNAc O-acetylation controls GspB glycosylation, such that binding via this adhesin is optimized. Moreover, because O-acetylation has comparable effects on the glycosylation of other SRR adhesins, acetylation may represent a conserved regulatory mechanism for the post-translational modification of the SRR glycoprotein family.
Identifiants
pubmed: 33384382
pii: S0021-9258(21)00016-8
doi: 10.1074/jbc.RA120.016116
pmc: PMC7948813
pii:
doi:
Substances chimiques
Glycoproteins
0
Membrane Proteins
0
Serine
452VLY9402
Glycosyltransferases
EC 2.4.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
100249Subventions
Organisme : NIAID NIH HHS
ID : R01 AI041513
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI106987
Pays : United States
Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.
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