Dental pulp stem cells from human teeth with deep caries displayed an enhanced angiogenesis potential
Angiogenesis
Deep caries
Dental pulp stem cells
Pulp regeneration
Journal
Journal of dental sciences
ISSN: 2213-8862
Titre abrégé: J Dent Sci
Pays: Netherlands
ID NLM: 101293181
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
11
02
2020
revised:
03
03
2020
entrez:
1
1
2021
pubmed:
2
1
2021
medline:
2
1
2021
Statut:
ppublish
Résumé
Dental pulp stem cells can be isolated from human teeth with deep caries (cDPSCs), but their biological characteristics are still unclear. The aim of this study was to investigate the angiogenic potential of cDPSCs and compare them to dental pulp stem cells from human normal teeth (nDPSCs). Cells were isolated from human pulp tissue of normal and infected teeth with deep caries. Basic mesenchymal stem cell (MSC) characterization was conducted. Colony forming units and proliferation ability were evaluated in nDPSCs and cDPSCs. Expression of VEGF in both tissues and cells was examined by immunohistochemical staining. After stimulating nDPSCs and cDPSCs with an angiogenic medium, angiogenic markers were evaluated by qRT-PCR and western blotting. Finally, tube formation assays were used to evaluate the Both nDPSCs and cDPSCs possessed typical MSC characteristics. cDPSCs had enhanced colony formation and proliferation capacities than nDPSCs did. The expression of VEGF was higher in pulp tissue from teeth with deep caries and cDPSCs than in normal tissue and nDPSCs. When both cell types were grown cDPSCs maintained MSC traits and presented a higher angiogenesis potential than nDPSCs.
Sections du résumé
BACKGROUND/PURPOSE
OBJECTIVE
Dental pulp stem cells can be isolated from human teeth with deep caries (cDPSCs), but their biological characteristics are still unclear. The aim of this study was to investigate the angiogenic potential of cDPSCs and compare them to dental pulp stem cells from human normal teeth (nDPSCs).
MATERIALS AND METHODS
METHODS
Cells were isolated from human pulp tissue of normal and infected teeth with deep caries. Basic mesenchymal stem cell (MSC) characterization was conducted. Colony forming units and proliferation ability were evaluated in nDPSCs and cDPSCs. Expression of VEGF in both tissues and cells was examined by immunohistochemical staining. After stimulating nDPSCs and cDPSCs with an angiogenic medium, angiogenic markers were evaluated by qRT-PCR and western blotting. Finally, tube formation assays were used to evaluate the
RESULTS
RESULTS
Both nDPSCs and cDPSCs possessed typical MSC characteristics. cDPSCs had enhanced colony formation and proliferation capacities than nDPSCs did. The expression of VEGF was higher in pulp tissue from teeth with deep caries and cDPSCs than in normal tissue and nDPSCs. When both cell types were grown
CONCLUSION
CONCLUSIONS
cDPSCs maintained MSC traits and presented a higher angiogenesis potential than nDPSCs.
Identifiants
pubmed: 33384815
doi: 10.1016/j.jds.2020.03.007
pii: S1991-7902(20)30055-6
pmc: PMC7770258
doi:
Types de publication
Journal Article
Langues
eng
Pagination
318-326Informations de copyright
© 2020 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.
Déclaration de conflit d'intérêts
The authors declare that they have no conflict of interest.
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