Glycation of glyceraldehyde-3-phosphate dehydrogenase inhibits the binding with α-synuclein and RNA.


Journal

Archives of biochemistry and biophysics
ISSN: 1096-0384
Titre abrégé: Arch Biochem Biophys
Pays: United States
ID NLM: 0372430

Informations de publication

Date de publication:
15 02 2021
Historique:
received: 29 07 2020
revised: 18 12 2020
accepted: 21 12 2020
pubmed: 2 1 2021
medline: 20 2 2021
entrez: 1 1 2021
Statut: ppublish

Résumé

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) shows great diversity of functions, interaction partners and post-translational modifications. GAPDH undergoes glycation of positively charged residues in diabetic patient's tissues and therefore may change interaction with partners. The influence of GAPDH glycation on interaction with two important partners, α-synuclein and RNA, has been investigated in silico using molecular dynamics simulations and in vitro using surface plasmon resonance measurements. Since positively charged groove including substrate- and NAD

Identifiants

pubmed: 33385367
pii: S0003-9861(20)30752-9
doi: 10.1016/j.abb.2020.108744
pii:
doi:

Substances chimiques

SNCA protein, human 0
alpha-Synuclein 0
RNA 63231-63-0
GAPDH protein, human EC 1.2.1.12
Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) EC 1.2.1.12

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

108744

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Alina A Sofronova (AA)

Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia.

Denis V Pozdyshev (DV)

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.

Kseniya V Barinova (KV)

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.

Vladimir I Muronetz (VI)

Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.

Pavel I Semenyuk (PI)

Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. Electronic address: psemenyuk@belozersky.msu.ru.

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Classifications MeSH