Metabolomics approach based on LC-HRMS for the fast screening of iron(II)-chelating peptides in protein hydrolysates.
Caseins
/ chemistry
Chelating Agents
/ chemistry
Chromatography, Liquid
/ methods
Electronic Data Processing
Hydrolysis
Iron
/ chemistry
Ligands
Limit of Detection
Mass Spectrometry
Metabolomics
/ methods
Peptides
/ chemistry
Protein Hydrolysates
/ chemistry
Proteins
/ chemistry
Spectrometry, Mass, Electrospray Ionization
Direct screening
Iron (II)
Metal-chelating peptides
Online LC-MS method
Protein hydrolysates
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
05
09
2020
accepted:
28
10
2020
revised:
21
10
2020
pubmed:
3
1
2021
medline:
7
9
2021
entrez:
2
1
2021
Statut:
ppublish
Résumé
Production of iron-chelating peptides from protein hydrolysates requires robust and adequate screening methods to optimize their purification and subsequently valorize their potential antioxidant properties. An original methodology was developed for direct and sensitive screening of iron(II)-chelating peptides based on ion-pair reverse phase liquid chromatography (IP-RPLC) coupled to high-resolution mass spectrometry (HRMS). Peptide mixture was first added to iron(II) solution to form iron(II)-peptide complexes. Then IP-RPLC-HRMS analysis was conducted on this iron-peptide mixture and on the iron-free peptide solution for comparative mass spectra analysis. This protocol, initially applied to a range of low molecular weight standard peptides, allowed detection of [(Peptide-H)+
Identifiants
pubmed: 33386417
doi: 10.1007/s00216-020-03037-1
pii: 10.1007/s00216-020-03037-1
doi:
Substances chimiques
Caseins
0
Chelating Agents
0
Ligands
0
Peptides
0
Protein Hydrolysates
0
Proteins
0
Iron
E1UOL152H7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
315-329Références
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