Anti-apoptotic effects of human gingival mesenchymal stromal cells on polymorphonuclear leucocytes.
gingiva
immunomodulation
mesenchymal stromal cells
periodontitis
polymorphonuclear leucocytes
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
Apr 2022
Apr 2022
Historique:
revised:
14
12
2020
received:
29
04
2020
accepted:
28
12
2020
pubmed:
3
1
2021
medline:
9
3
2022
entrez:
2
1
2021
Statut:
ppublish
Résumé
Polymorphonuclear leucocytes (PMNs) constitute the first line of host defence and are crucial in maintaining periodontal health. Their survival and function are modulated by mesenchymal stromal cells (MSCs) from different origin. Gingival MSCs (GMSCs) play an important role in maintaining oral health and in the initial inflammatory response. The present study aimed to investigate the effects of GMSCs on PMNs apoptosis and reactive oxygen species (ROS) production. PMNs were either directly incubated with untreated, interleukin (IL)-1β- or tumour necrosis factor (TNF)-α-treated GMSCs or stimulated with their conditioned media. Resulting ROS production was evaluated by dichlorofluorescin diacetate staining, whereas PMNs apoptosis was assessed by Annexin V staining, followed by flow cytometry analysis. While conditioned media of untreated and TNF-α-treated GMSCs did not affect apoptosis of PMNs, it was significantly delayed by conditioned media of GMSCs treated with IL-1β. In direct co-culture, GMSCs exerted anti-apoptotic effects on PMNs independently of the previous stimulation. However, the strongest impact was observed by IL-1β-treated GMSCs. ROS production of PMNs was not influenced by GMSCs or their conditioned media. This study demonstrates for the first time the immunomodulatory properties of GMSCs towards PMNs, revealing that IL-1β enhances anti-apoptotic effects of GMSCs.
Identifiants
pubmed: 33386669
doi: 10.1111/odi.13768
pmc: PMC9290793
doi:
Substances chimiques
Culture Media, Conditioned
0
Types de publication
Journal Article
Langues
eng
Pagination
777-785Subventions
Organisme : Austrian Science Fund FWF
ID : P 29440
Pays : Austria
Organisme : Medizinisch-Wissenschaftlicher Fonds des Bürgermeisters der Bundeshauptstadt Wien
ID : 19010
Organisme : Austrian Science Fund
ID : P29440
Informations de copyright
© 2021 The Authors. Oral Diseases published by Wiley Periodicals LLC.
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