Inhibition of the replication of SARS-CoV-2 in human cells by the FDA-approved drug chlorpromazine.
COVID-19
Chlorpromazine
Human cells
Repurposing of molecules
SARS-CoV-2
Journal
International journal of antimicrobial agents
ISSN: 1872-7913
Titre abrégé: Int J Antimicrob Agents
Pays: Netherlands
ID NLM: 9111860
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
15
06
2020
revised:
14
12
2020
accepted:
20
12
2020
pubmed:
3
1
2021
medline:
23
3
2021
entrez:
2
1
2021
Statut:
ppublish
Résumé
Urgent action is needed to fight the ongoing coronavirus disease 2019 (COVID-19) pandemic by reducing the number of infected cases, contagiousness and severity. Chlorpromazine (CPZ), an antipsychotic from the phenothiazine group, is known to inhibit clathrin-mediated endocytosis and has antiviral activity against severe acute respiratory syndrome coronavirus-1 (SARS-CoV-1) and Middle East respiratory syndrome coronavirus. The aim of this in-vitro study was to test CPZ against SARS-CoV-2 in monkey and human cells. Monkey VeroE6 cells and human alveolar basal epithelial A549-ACE2 cells were infected with SARS-CoV-2 in the presence of various concentrations of CPZ. Supernatants were harvested at day 2 and analysed by quantitative reverse transcription polymerase chain reaction (RT-qPCR) for the presence of SARS-CoV-2 RNA. Cell viability was assessed in non-infected cells. CPZ was found to have antiviral activity against SARS-CoV-2 in monkey VeroE6 cells, with a half maximal inhibitory concentration (IC Although the measured SI values are low, the IC These preclinical findings support clinical investigation of the repurposing of CPZ, a drug with mild side effects, in the treatment of patients with COVID-19.
Identifiants
pubmed: 33387629
pii: S0924-8579(20)30502-1
doi: 10.1016/j.ijantimicag.2020.106274
pmc: PMC7772996
pii:
doi:
Substances chimiques
Antiviral Agents
0
Chlorpromazine
U42B7VYA4P
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
106274Informations de copyright
Copyright © 2021 Elsevier Ltd. All rights reserved.
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