Characterization of exosomal long non-coding RNAs in chondrogenic differentiation of human adipose-derived stem cells.
Adipocytes
/ metabolism
Adipose Tissue
/ cytology
Cartilage
/ pathology
Cell Differentiation
Cells, Cultured
Chondrocytes
/ cytology
Chondrogenesis
Exosomes
/ metabolism
Gene Expression Profiling
Gene Expression Regulation
Gene Regulatory Networks
Humans
MicroRNAs
/ genetics
RNA, Long Noncoding
RNA, Messenger
/ metabolism
Sequence Analysis, RNA
Stem Cells
/ cytology
Chondrogenesis
Exosomes
LncRNAs
Journal
Molecular and cellular biochemistry
ISSN: 1573-4919
Titre abrégé: Mol Cell Biochem
Pays: Netherlands
ID NLM: 0364456
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
12
06
2020
accepted:
24
11
2020
pubmed:
4
1
2021
medline:
3
8
2021
entrez:
3
1
2021
Statut:
ppublish
Résumé
The exosomes derived from chondrogenic stem cells and long non-coding RNAs (lncRNAs) play a key role in cartilage regeneration. Here, we investigated the expression profile of exosomal lncRNAs in chondrogenesis of human adipose derived stem cells (hADSCs). hADSCs were induced to differentiate into chondrocytes in vitro. Exosomes from undifferentiated hADSCs and chondrogenic hADSCs were isolated. LncRNA and mRNA expression profiles in the isolated exosomes were analyzed by RNA sequencing. The resultant data were subjected to gene ontology (GO) terms and KEGG pathway analysis to identify differentially expressed lncRNAs. We identified 23 upregulated and 163 downregulated lncRNAs in exosomes derived from chondrogenic hADSCs compared to that in exosomes from undifferentiated hADSCs. In addition, analysis of mRNA expression data revealed 968 upregulated genes and 572 downregulated genes in exosomes of chondrogenic hADSCs. Lncrna and mRNA expression levels were further validated by qRT-PCR. Differentially expressed lncRNAs and mRNAs were utilized to construct a coding-non-coding gene co-expression network (CNC network). GO terms and KEGG pathway enrichment analysis revealed several significant processes differentially regulated between undifferentiated hADSCs and chondrogenic hADSCs. Taken together, this study revealed the differential expression of exosomal lncRNAs of chondrogenic hADSCs and provided a foundation for future study on the cartilage recovery mechanism of exosomes derived from chondrogenic stem cells.
Identifiants
pubmed: 33389494
doi: 10.1007/s11010-020-04003-2
pii: 10.1007/s11010-020-04003-2
doi:
Substances chimiques
MicroRNAs
0
RNA, Long Noncoding
0
RNA, Messenger
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1411-1420Subventions
Organisme : National Natural Science Foundation of China
ID : 81874014
Organisme : National Natural Science Foundation of China
ID : 81672198
Organisme : National Natural Science Foundation of China
ID : 81802187
Organisme : Natural Science Foundation of Guangdong Province
ID : 2018030310355
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