Single-cell profiling of the human decidual immune microenvironment in patients with recurrent pregnancy loss.
Journal
Cell discovery
ISSN: 2056-5968
Titre abrégé: Cell Discov
Pays: England
ID NLM: 101661034
Informations de publication
Date de publication:
04 Jan 2021
04 Jan 2021
Historique:
received:
04
09
2020
accepted:
23
11
2020
entrez:
4
1
2021
pubmed:
5
1
2021
medline:
5
1
2021
Statut:
epublish
Résumé
Maintaining homeostasis of the decidual immune microenvironment at the maternal-fetal interface is essential for placentation and reproductive success. Although distinct decidual immune cell subpopulations have been identified under normal conditions, systematic understanding of the spectrum and heterogeneity of leukocytes under recurrent miscarriage in human deciduas remains unclear. To address this, we profiled the respective transcriptomes of 18,646 primary human decidual immune cells isolated from patients with recurrent pregnancy loss (RPL) and healthy controls at single-cell resolution. We discovered dramatic differential distributions of immune cell subsets in RPL patients compared with the normal decidual immune microenvironment. Furthermore, we found a subset of decidual natural killer (NK) cells that support embryo growth were diminished in proportion due to abnormal NK cell development in RPL patients. We also elucidated the altered cellular interactions between the decidual immune cell subsets in the microenvironment and those of the immune cells with stromal cells and extravillous trophoblast under disease state. These results provided deeper insights into the RPL decidual immune microenvironment disorder that are potentially applicable to improve the diagnosis and therapeutics of this disease.
Identifiants
pubmed: 33390590
doi: 10.1038/s41421-020-00236-z
pii: 10.1038/s41421-020-00236-z
pmc: PMC7779601
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1Subventions
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 81788101
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 31970858
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 31771428
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 91940306
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 91640113
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 31700796
Organisme : National Natural Science Foundation of China (National Science Foundation of China)
ID : 81871479
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