Ouabain Promotes Gap Junctional Intercellular Communication in Cancer Cells.
cancer
gap junctions
ouabain
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
31 Dec 2020
31 Dec 2020
Historique:
received:
13
09
2020
revised:
01
12
2020
accepted:
03
12
2020
entrez:
5
1
2021
pubmed:
6
1
2021
medline:
26
3
2021
Statut:
epublish
Résumé
Gap junctions are molecular structures that allow communication between neighboring cells. It has been shown that gap junctional intercellular communication (GJIC) is notoriously reduced in cancer cells compared to their normal counterparts. Ouabain, a plant derived substance, widely known for its therapeutic properties on the heart, has been shown to play a role in several types of cancer, although its mechanism of action is not yet fully understood. Since we have previously shown that ouabain enhances GJIC in epithelial cells (MDCK), here we probed whether ouabain affects GJIC in a variety of cancer cell lines, including cervico-uterine (CasKi, SiHa and Hela), breast (MDA-MB-321 and MCF7), lung (A549), colon (SW480) and pancreas (HPAF-II). For this purpose, we conducted dye transfer assays to measure and compare GJIC in monolayers of cells with and without treatment with ouabain (0.1, 1, 10, 50 and 500 nM). We found that ouabain induces a statistically significant enhancement of GJIC in all of these cancer cell lines, albeit with distinct sensitivity. Additionally, we show that synthesis of new nucleotides or protein subunits is not required, and that Csrc, ErK1/2 and ROCK-Rho mediate the signaling mechanisms. These results may contribute to explaining how ouabain influences cancer.
Identifiants
pubmed: 33396341
pii: ijms22010358
doi: 10.3390/ijms22010358
pmc: PMC7801950
pii:
doi:
Substances chimiques
Cardiotonic Agents
0
Ouabain
5ACL011P69
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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