Standardization of ELISA protocols for serosurveys of the SARS-CoV-2 pandemic using clinical and at-home blood sampling.

Antibodies, Viral / blood COVID-19 / blood COVID-19 Nucleic Acid Testing COVID-19 Serological Testing / methods COVID-19 Testing Enzyme-Linked Immunosorbent Assay / methods Humans Immunoglobulin G / blood Immunoglobulin M / blood Pandemics Reference Standards SARS-CoV-2 / immunology Sensitivity and Specificity Spike Glycoprotein, Coronavirus / immunology

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
04 01 2021
Historique:
received: 24 06 2020
accepted: 29 11 2020
entrez: 5 1 2021
pubmed: 6 1 2021
medline: 14 1 2021
Statut: epublish

Résumé

The extent of SARS-CoV-2 infection throughout the United States population is currently unknown. High quality serology is key to avoiding medically costly diagnostic errors, as well as to assuring properly informed public health decisions. Here, we present an optimized ELISA-based serology protocol, from antigen production to data analyses, that helps define thresholds for IgG and IgM seropositivity with high specificities. Validation of this protocol is performed using traditionally collected serum as well as dried blood on mail-in blood sampling kits. Archival (pre-2019) samples are used as negative controls, and convalescent, PCR-diagnosed COVID-19 patient samples serve as positive controls. Using this protocol, minimal cross-reactivity is observed for the spike proteins of MERS, SARS1, OC43 and HKU1 viruses, and no cross reactivity is observed with anti-influenza A H1N1 HAI. Our protocol may thus help provide standardized, population-based data on the extent of SARS-CoV-2 seropositivity, immunity and infection.

Identifiants

DOI: 10.1038/s41467-020-20383-x PMID: 33397956 PMC: PMC7782755
pubmed: 33397956
doi: 10.1038/s41467-020-20383-x
pii: 10.1038/s41467-020-20383-x
pmc: PMC7782755
doi:

Substances chimiques

Antibodies, Viral 0
Immunoglobulin G 0
Immunoglobulin M 0
Spike Glycoprotein, Coronavirus 0
spike protein, SARS-CoV-2 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

113

Subventions

Organisme : CCR NIH HHS
ID : HHSN261200800001C
Pays : United States
Organisme : NCI NIH HHS
ID : HHSN261200800001E
Pays : United States

Commentaires et corrections

Type : UpdateOf

Références

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Auteurs

Carleen Klumpp-Thomas (C)

National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, 20850, USA.
Section on Immuno-Engineering, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, 20894, USA.
ORCID: 0000-0002-6646-6132

Heather Kalish (H)

Trans-NIH Shared Resource on Biomedical Engineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, 20894, USA.

Matthew Drew (M)

Protein Expression Laboratory, NCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.

Sally Hunsberger (S)

Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 20894, USA.

Kelly Snead (K)

Protein Expression Laboratory, NCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.

Michael P Fay (MP)

Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 20894, USA.
ORCID: 0000-0002-8643-9625

Jennifer Mehalko (J)

Protein Expression Laboratory, NCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.

Anandakumar Shunmugavel (A)

Section on Immuno-Engineering, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, 20894, USA.

Vanessa Wall (V)

Protein Expression Laboratory, NCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.

Peter Frank (P)

Protein Expression Laboratory, NCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.

John-Paul Denson (JP)

Protein Expression Laboratory, NCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.

Min Hong (M)

Protein Expression Laboratory, NCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.

Gulcin Gulten (G)

Protein Expression Laboratory, NCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.

Simon Messing (S)

Protein Expression Laboratory, NCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.

Jennifer Hicks (J)

Trans-NIH Shared Resource on Biomedical Engineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, 20894, USA.

Sam Michael (S)

National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, 20850, USA.

William Gillette (W)

Protein Expression Laboratory, NCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.

Matthew D Hall (MD)

National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, 20850, USA.

Matthew J Memoli (MJ)

LID Clinical Studies Unit, Laboratory of Infectious Diseases, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 20894, USA.
ORCID: 0000-0001-6393-5178

Dominic Esposito (D)

Protein Expression Laboratory, NCI RAS Initiative, Frederick National Laboratory for Cancer Research, Frederick, MD, 21702, USA.

Kaitlyn Sadtler (K)

Section on Immuno-Engineering, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, 20894, USA. kaitlyn.sadtler@nih.gov.
ORCID: 0000-0003-2587-8330

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps antiviraux › Standardization of ELISA protocols for...
questionsmedicales.fr › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Pneumopathie infectieuse › Pneumopathie virale › COVID-19 › Standardization of ELISA protocols for...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Dépistage de la COVID-19 › Détection de l'acide nucléique du virus de la COVID-19 › Standardization of ELISA protocols for...
questionsmedicales.fr › Techniques d'investigation › Techniques immunologiques › Tests immunologiques › Tests sérologiques › Dépistage sérologique de la COVID-19 › Standardization of ELISA protocols for...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Dépistage de la COVID-19 › Standardization of ELISA protocols for...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Techniques d'immunoadsorption › Test ELISA › Standardization of ELISA protocols for...
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questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Isotypes des immunoglobulines › Immunoglobuline M › Standardization of ELISA protocols for...
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