Insulin-Like Growth Factor Binding Protein-3 Binds to Histone 3.
IGFBP-3
histone 3
protein-protein interaction
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
02 Jan 2021
02 Jan 2021
Historique:
received:
05
12
2020
revised:
25
12
2020
accepted:
29
12
2020
entrez:
6
1
2021
pubmed:
7
1
2021
medline:
14
4
2021
Statut:
epublish
Résumé
Insulin-like growth factor (IGF) binding protein-3 (IGFBP-3) is an essential protein that regulates cellular processes such as cell proliferation, apoptosis, and differentiation. It is known to bind with several proteins to carry out various cellular functions. In this study, we report for the first time that IGFBP-3 is a histone 3 (H3) binding protein. Sub-cellular fractionation was performed to separate into cytosolic fraction, nucleic acid binding protein fraction and insoluble nuclear fraction. Using ligand blot analysis, we identified a ~15 kDa protein that can interact with IGFBP-3 in the insoluble nuclear fraction. The 15 kDa protein was confirmed as histone 3 by far-Western blot analysis and co-immunoprecipitation experiments. A dot-blot experiment further validated the binding of IGFBP-3 with H3. The intensity of IGFBP-3 on dot-blot showed a proportional increase with H3 concentrations between 2.33 pmol-37.42 pmol. Our results support the presence of protein-protein interaction between IGFBP-3 and H3. The physical binding between IGFBP-3 and H3 could indicate its yet another cellular role in regulating the chromatin remodeling for gene transcription.
Identifiants
pubmed: 33401705
pii: ijms22010407
doi: 10.3390/ijms22010407
pmc: PMC7796407
pii:
doi:
Substances chimiques
Histones
0
Insulin-Like Growth Factor Binding Protein 3
0
Ligands
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Manitoba Medical Service Foundation
ID : MMSF, Grant #8-2017-08
Organisme : Department of Surgery Grant, University of Manitoba
ID : UM-FOP 31763-342100-2000
Organisme : Major Grant, University of Winnipeg
ID : Grant ID 10390
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