Hyaluronidase Impairs Neutrophil Function and Promotes Group B
Amniotic Fluid
/ microbiology
Animals
Cytokines
/ metabolism
Disease Models, Animal
Female
Humans
Hyaluronoglucosaminidase
/ genetics
Inflammation
Lung
/ microbiology
Macaca nemestrina
Neutrophils
/ immunology
Obstetric Labor, Premature
/ immunology
Pregnancy
Premature Birth
Primates
Streptococcal Infections
/ immunology
Streptococcus agalactiae
/ enzymology
group B streptococcus
hyaluronidase
immune evasion
neutrophils
pregnancy
preterm labor
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
05 01 2021
05 01 2021
Historique:
entrez:
6
1
2021
pubmed:
7
1
2021
medline:
9
2
2021
Statut:
epublish
Résumé
Invasive bacterial infections during pregnancy are a major risk factor for preterm birth, stillbirth, and fetal injury. Group B streptococci (GBS) are Gram-positive bacteria that asymptomatically colonize the lower genital tract but infect the amniotic fluid and induce preterm birth or stillbirth. Experimental models that closely emulate human pregnancy are pivotal for the development of successful strategies to prevent these adverse pregnancy outcomes. Using a unique nonhuman primate model that mimics human pregnancy and informs temporal events surrounding amniotic cavity invasion and preterm labor, we show that the animals inoculated with hyaluronidase (HylB)-expressing GBS consistently exhibited microbial invasion into the amniotic cavity, fetal bacteremia, and preterm labor. Although delayed cytokine responses were observed at the maternal-fetal interface, increased prostaglandin and matrix metalloproteinase levels in these animals likely mediated preterm labor. HylB-proficient GBS dampened reactive oxygen species production and exhibited increased resistance to neutrophils compared to an isogenic mutant. Together, these findings demonstrate how a bacterial enzyme promotes GBS amniotic cavity invasion and preterm labor in a model that closely resembles human pregnancy.
Identifiants
pubmed: 33402537
pii: mBio.03115-20
doi: 10.1128/mBio.03115-20
pmc: PMC8545101
pii:
doi:
Substances chimiques
Cytokines
0
Hyaluronoglucosaminidase
EC 3.2.1.35
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : T32 AI007509
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI145890
Pays : United States
Organisme : NIH HHS
ID : P51 OD010425
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI133976
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI100989
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI112619
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI055396
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK017047
Pays : United States
Informations de copyright
Copyright © 2021 Coleman et al.
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