Serological Proteome Analysis for the Characterization of Secreted Fungal Protein Antigens.
Antibody
Aspergillus fumigatus
Fungal pathogens
Humoral response
Immunoglobulin
Immunoproteomics
SERPA
Secretome
Serological proteome analysis
Western blot
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
6
1
2021
pubmed:
7
1
2021
medline:
1
4
2021
Statut:
ppublish
Résumé
Defining the humoral immune response to infectious agents is important for gaining insights into infectious diseases and the response of the immune system. It can further aid development of serodiagnostic tests, discovery of vaccine antigen candidates, and immuno-epidemiological research. During the last three decades, serological proteome analyses (SERPAs) have played a significant role in characterizing the antibody response of humans or animals to fungal pathogens. SERPA combines 2D-gel electrophoresis with Western blotting. The introduction of multiplexing approaches by means of fluorescent dyes has greatly improved the reliability of the 2D technique and has boosted also the qualitative capabilities of the SERPA approach. In this chapter, we detail a SERPA protocol using fungal extracellular proteins from a fungal culture, here as an example the mold Aspergillus fumigatus.
Identifiants
pubmed: 33405028
doi: 10.1007/978-1-0716-1182-1_2
doi:
Substances chimiques
Antigens, Fungal
0
Fungal Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
15-26Références
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