Direct comparison of 3D and 2D cultivation reveals higher osteogenic capacity of elderly osteoblasts in 3D.
Aged
Aged, 80 and over
Aging
/ genetics
Cell Culture Techniques
/ methods
Cell Differentiation
/ genetics
Cells, Cultured
Collagen Type I
/ genetics
Female
Fracture Healing
/ physiology
Gene Expression
Humans
Male
Osteoblasts
/ metabolism
Osteocalcin
/ genetics
Osteogenesis
/ genetics
Osteopontin
/ genetics
Osteoprotegerin
/ genetics
2D/3D cultivation
Donor age
Osteoblasts
Journal
Journal of orthopaedic surgery and research
ISSN: 1749-799X
Titre abrégé: J Orthop Surg Res
Pays: England
ID NLM: 101265112
Informations de publication
Date de publication:
06 Jan 2021
06 Jan 2021
Historique:
received:
16
11
2020
accepted:
09
12
2020
entrez:
7
1
2021
pubmed:
8
1
2021
medline:
13
7
2021
Statut:
epublish
Résumé
The aim of this study was the investigation of the osteogenic potential of human osteoblasts of advanced donor age in 2D and 3D culture. Osteoblasts were induced to osteogenic differentiation and cultivated, using the same polystyrene material in 2D and 3D culture for 2 weeks. Samples were taken to evaluate alkaline phosphatase (ALP) activity, mineralization and gene expression. Osteoprotegerin (OPG) levels were significantly increased (8.2-fold) on day 7 in 3D compared to day 0 (p < 0.0001) and 11.6-fold higher in 3D than in 2D (p < 0.0001). Both culture systems showed reduced osteocalcin (OC) levels (2D 85% and 3D 50% of basic value). Collagen type 1 (Col1) expression was elevated in 3D on day 7 (1.4-fold; p = 0.009). Osteopontin (OP) expression showed 6.5-fold higher levels on day 7 (p = 0.002) in 3D than in 2D. Mineralization was significantly higher in 3D on day 14 (p = 0.0002). Advanced donor age human primary osteoblasts reveal significantly higher gene expression levels of OPG, Col1 and OP in 3D than in monolayer. Therefore, it seems that a relatively high potential of bone formation in a natural 3D arrangement is presumably still present in osteoblasts of elderly people. 5217/11 on the 22nd of Dec. 2011.
Sections du résumé
BACKGROUND
BACKGROUND
The aim of this study was the investigation of the osteogenic potential of human osteoblasts of advanced donor age in 2D and 3D culture.
METHODS
METHODS
Osteoblasts were induced to osteogenic differentiation and cultivated, using the same polystyrene material in 2D and 3D culture for 2 weeks. Samples were taken to evaluate alkaline phosphatase (ALP) activity, mineralization and gene expression.
RESULTS
RESULTS
Osteoprotegerin (OPG) levels were significantly increased (8.2-fold) on day 7 in 3D compared to day 0 (p < 0.0001) and 11.6-fold higher in 3D than in 2D (p < 0.0001). Both culture systems showed reduced osteocalcin (OC) levels (2D 85% and 3D 50% of basic value). Collagen type 1 (Col1) expression was elevated in 3D on day 7 (1.4-fold; p = 0.009). Osteopontin (OP) expression showed 6.5-fold higher levels on day 7 (p = 0.002) in 3D than in 2D. Mineralization was significantly higher in 3D on day 14 (p = 0.0002).
CONCLUSION
CONCLUSIONS
Advanced donor age human primary osteoblasts reveal significantly higher gene expression levels of OPG, Col1 and OP in 3D than in monolayer. Therefore, it seems that a relatively high potential of bone formation in a natural 3D arrangement is presumably still present in osteoblasts of elderly people.
TRIAL REGISTRATION
BACKGROUND
5217/11 on the 22nd of Dec. 2011.
Identifiants
pubmed: 33407623
doi: 10.1186/s13018-020-02153-z
pii: 10.1186/s13018-020-02153-z
pmc: PMC7788858
doi:
Substances chimiques
Collagen Type I
0
Osteoprotegerin
0
Osteocalcin
104982-03-8
Osteopontin
106441-73-0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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