A novel fluorescence immunochromatographic assay strip for the diagnosis of schistosomiasis japonica.
Animals
Animals, Domestic
/ immunology
Antibodies, Helminth
/ blood
Antigens, Helminth
/ immunology
Bacterial Proteins
/ immunology
Enzyme-Linked Immunosorbent Assay
/ methods
Fluorescent Dyes
Immunoassay
/ methods
Mice
Recombinant Proteins
/ immunology
Schistosoma japonicum
/ immunology
Schistosomiasis japonica
/ diagnosis
ELISA
Fluorescence immunochromatographic assay
Schistosoma japonicum
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
06 Jan 2021
06 Jan 2021
Historique:
received:
08
11
2019
accepted:
01
12
2020
entrez:
7
1
2021
pubmed:
8
1
2021
medline:
12
5
2021
Statut:
epublish
Résumé
Schistosomiasis japonica is a severe zoonosis. Domestic animals are the primary source of infection and play an important role in disease transmission. Surveillance and diagnosis play key roles in schistosomiasis control; however, current techniques for the surveillance and diagnosis of the disease have limitations. In this study, we developed a novel fluorescence immunochromatographic assay (FICA) strip to detect anti-Schistosoma japonicum antibodies in host serum. A FICA strip was developed for the diagnosis of Schistosoma japonicum in domestic animals. Streptococcus protein G (SPG) and soluble egg antigen (SEA) were transferred onto a nitrocellulose (NC) membrane to form the control line (C) and the test line (T), respectively. With fluorescence activity as well as binding activity to multispecies IgG, the recombinant protein rSPG-RFP was expressed and employed as an antibody indicator in the FICA strips. The dual gene fusion plasmid was verified by PCR and restriction enzyme digestion. The expressed recombinant protein was 39.72 kDa in size, which was consistent with the predicted molecular weight. The western blot results showed binding activity between rSPG-RFP and IgGs from different hosts. Fluorescence microscopy also showed the fluorescence activity of the protein present. The affinity constant (Ka) values of rSPG-RFP with rabbit, donkey, mouse and goat IgG were 1.9 × 10 Based on the rSPG-RFP protein that we developed, strip detection can be completed within 15 min. Heightened sensitivity allows the strip to accurately identify schistosome antibodies in serum. In conclusion, this method is convenient, feasible, rapid and effective for detecting S. japonicum.
Sections du résumé
BACKGROUND
BACKGROUND
Schistosomiasis japonica is a severe zoonosis. Domestic animals are the primary source of infection and play an important role in disease transmission. Surveillance and diagnosis play key roles in schistosomiasis control; however, current techniques for the surveillance and diagnosis of the disease have limitations. In this study, we developed a novel fluorescence immunochromatographic assay (FICA) strip to detect anti-Schistosoma japonicum antibodies in host serum.
METHODS
METHODS
A FICA strip was developed for the diagnosis of Schistosoma japonicum in domestic animals. Streptococcus protein G (SPG) and soluble egg antigen (SEA) were transferred onto a nitrocellulose (NC) membrane to form the control line (C) and the test line (T), respectively. With fluorescence activity as well as binding activity to multispecies IgG, the recombinant protein rSPG-RFP was expressed and employed as an antibody indicator in the FICA strips.
RESULTS
RESULTS
The dual gene fusion plasmid was verified by PCR and restriction enzyme digestion. The expressed recombinant protein was 39.72 kDa in size, which was consistent with the predicted molecular weight. The western blot results showed binding activity between rSPG-RFP and IgGs from different hosts. Fluorescence microscopy also showed the fluorescence activity of the protein present. The affinity constant (Ka) values of rSPG-RFP with rabbit, donkey, mouse and goat IgG were 1.9 × 10
CONCLUSIONS
CONCLUSIONS
Based on the rSPG-RFP protein that we developed, strip detection can be completed within 15 min. Heightened sensitivity allows the strip to accurately identify schistosome antibodies in serum. In conclusion, this method is convenient, feasible, rapid and effective for detecting S. japonicum.
Identifiants
pubmed: 33407752
doi: 10.1186/s13071-020-04511-6
pii: 10.1186/s13071-020-04511-6
pmc: PMC7788720
doi:
Substances chimiques
Antibodies, Helminth
0
Antigens, Helminth
0
Bacterial Proteins
0
Fluorescent Dyes
0
IgG Fc-binding protein, Streptococcus
0
Recombinant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8Subventions
Organisme : The National Key Research and Development Program of China
ID : 2017YFD0501306
Organisme : Basal Research Fund
ID : 0201007002008
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