Oxidative stress and DNA damage in agricultural workers after exposure to pesticides.

Agricultural workers Biomonitoring Cancer Disease Pesticides ROS

Journal

Journal of occupational medicine and toxicology (London, England)
ISSN: 1745-6673
Titre abrégé: J Occup Med Toxicol
Pays: England
ID NLM: 101245790

Informations de publication

Date de publication:
07 Jan 2021
Historique:
received: 24 05 2020
accepted: 08 12 2020
entrez: 8 1 2021
pubmed: 9 1 2021
medline: 9 1 2021
Statut: epublish

Résumé

Recent epidemiological studies on workers describe that exposure to pesticides can induce oxidative stress by increased production of free radicals that can accumulate in the cell and damage biological macromolecules, for example, RNA, DNA, DNA repair proteins and other proteins and/or modify antioxidant defense mechanisms, as well as detoxification and scavenger enzymes. This study aimed to assess oxidative stress and DNA damage among workers exposed to pesticides. For this purpose, 52 pesticide exposed workers and 52 organic farmers were enrolled. They were assessed: the pesticide exposure, thiobarbituric acid reactive substances (TBARS), total glutathione (TG), oxidized glutathione levels (GSSG), and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), levels. Correlation between pesticide exposure was positively associated with high TBARS and 8-oxodG levels (p <  0.001). A negative association was founded with TG and GSSG and pesticide exposure. The present investigation results seem to indicate a mild augment in oxidative stress associated with pesticide exposure, followed by an adaptive response to increase the antioxidant defenses to prevent sustained oxidative adverse effects stress.

Sections du résumé

BACKGROUND BACKGROUND
Recent epidemiological studies on workers describe that exposure to pesticides can induce oxidative stress by increased production of free radicals that can accumulate in the cell and damage biological macromolecules, for example, RNA, DNA, DNA repair proteins and other proteins and/or modify antioxidant defense mechanisms, as well as detoxification and scavenger enzymes. This study aimed to assess oxidative stress and DNA damage among workers exposed to pesticides.
METHODS METHODS
For this purpose, 52 pesticide exposed workers and 52 organic farmers were enrolled. They were assessed: the pesticide exposure, thiobarbituric acid reactive substances (TBARS), total glutathione (TG), oxidized glutathione levels (GSSG), and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), levels.
RESULTS RESULTS
Correlation between pesticide exposure was positively associated with high TBARS and 8-oxodG levels (p <  0.001). A negative association was founded with TG and GSSG and pesticide exposure.
CONCLUSIONS CONCLUSIONS
The present investigation results seem to indicate a mild augment in oxidative stress associated with pesticide exposure, followed by an adaptive response to increase the antioxidant defenses to prevent sustained oxidative adverse effects stress.

Identifiants

pubmed: 33413467
doi: 10.1186/s12995-020-00290-z
pii: 10.1186/s12995-020-00290-z
pmc: PMC7791774
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1

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Auteurs

Caterina Ledda (C)

Department of Clinical and Experimental Medicine, Occupational Medicine, University of Catania, Via Santa Sofia, 89 - 95123, Catania, Italy. cledda@unict.it.

Emanuele Cannizzaro (E)

Department of Sciences for Health Promotion and Mother and Child Care, Occupational Medicine, University of Palermo, Palermo, Italy.

Diana Cinà (D)

Clinical Pathology and Clinical Molecular Biology Unit, "Garibaldi Centro" Hospital, ARNAS Garibaldi, Catania, Italy.

Vera Filetti (V)

Department of Clinical and Experimental Medicine, Occupational Medicine, University of Catania, Via Santa Sofia, 89 - 95123, Catania, Italy.

Ermanno Vitale (E)

Department of Clinical and Experimental Medicine, Occupational Medicine, University of Catania, Via Santa Sofia, 89 - 95123, Catania, Italy.

Gianluca Paravizzini (G)

Clinical Laboratories "Girlando and Paravizzini", Catania, Italy.

Concettina Di Naso (C)

Clinical Pathology and Clinical Molecular Biology Unit, "Garibaldi Centro" Hospital, ARNAS Garibaldi, Catania, Italy.

Ivo Iavicoli (I)

Department of Public Health, Occupational Medicine, University of Naples Federico II, Naples, Italy.

Venerando Rapisarda (V)

Department of Clinical and Experimental Medicine, Occupational Medicine, University of Catania, Via Santa Sofia, 89 - 95123, Catania, Italy.

Classifications MeSH