PCR-based diagnosis is not always useful in the acute acquired toxoplasmosis in immunocompetent individuals.
Acute acquired toxoplasmosis
Immunocompetent
PCR
Peripheral blood
Toxoplasma gondii
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
28
03
2020
accepted:
14
12
2020
pubmed:
9
1
2021
medline:
16
4
2021
entrez:
8
1
2021
Statut:
ppublish
Résumé
Toxoplasmosis is the most prevalent zoonosis in the world and is associated with a large spectrum of diseases. Acute acquired toxoplasmosis (AAT) is considered a benign and self-limiting disease but severe postnatal infections have been reported, particularly in South America. Laboratory diagnosis is based on the detection of anti-Toxoplasma gondii IgM, IgG, and presence of low IgG avidity. However, these assays present limitations, and therefore, PCR has been suggested as an alternative diagnostic tool. In this study, we performed real-time and nested PCR in DNA blood samples from 59 individuals with AAT lasting less than 80 days. None of the patients had parasitic DNA detected by PCR, even in the more severe cases or when blood was collected early after disease onset. These negative results indicate that the parasitemia kinetics needs investigation to determine the best time for blood sampling, especially in immunocompetent individuals. Thus, we emphasize that a negative PCR result does not exclude recent T. gondii infection, and serological criteria are still decisive for the laboratory diagnosis of AAT.
Identifiants
pubmed: 33415403
doi: 10.1007/s00436-020-07022-6
pii: 10.1007/s00436-020-07022-6
doi:
Substances chimiques
DNA, Protozoan
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
763-767Références
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