Inhibitory and ameliorative effect of heliomycin derived from actinomycete on induced hepatocellular carcinoma in rats.
Actinobacteria
/ metabolism
Alanine Transaminase
/ blood
Animals
Anticarcinogenic Agents
/ isolation & purification
Aspartate Aminotransferases
/ blood
Carcinoma, Hepatocellular
/ prevention & control
Diethylnitrosamine
Liver Neoplasms, Experimental
/ prevention & control
Male
Polycyclic Compounds
/ isolation & purification
Rats
Rats, Wistar
Time Factors
alpha-Fetoproteins
/ metabolism
Heliomycin
Hepatocellular carcinoma
Histopathology
Immunohistochemistry
Liver enzymes
Journal
Naunyn-Schmiedeberg's archives of pharmacology
ISSN: 1432-1912
Titre abrégé: Naunyn Schmiedebergs Arch Pharmacol
Pays: Germany
ID NLM: 0326264
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
22
09
2020
accepted:
13
12
2020
pubmed:
9
1
2021
medline:
15
12
2021
entrez:
8
1
2021
Statut:
ppublish
Résumé
The hepatoprotective activity of heliomycin obtained from the culture broth of actinomycete AB5 against diethylnitrosamine (DEN)-induced hepatic cancer in Wistar rats was estimated. Heliomycin exhibited a significant decrease in the levels of alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) compared to the positive control. For instance, the heliomycin group after 20 weeks showed a significant decline in ALT, AST, and ALP values (70.75 ± 5.12, 140.25 ± 11.75, and 163.25 ± 18.66, respectively) compared to the positive control group (170.00 ± 9.55, 252.75 ± 12.33, and 278.00 ± 21.32, respectively). Additionally, the isolated compound showed a highly significant decrease in serum alpha-fetoprotein (AFP) levels. After 8, 16, and 20 weeks, the mean values of AFP in the heliomycin group revealed a highly significant decrease (33.62 ± 2.46, 30.00 ± 4.05, and 28.50 ± 2.64, respectively) compared to the positive control group (49.45 ± 3.03, 81.90 ± 6.70, and 90.75 ± 5.12, respectively). The histopathological investigation of liver sections supported the results of biochemical analysis. It was demonstrated that heliomycin showed histological improvement of hepatocytes and marked increase of nuclear pyknotic with clear cytoplasm, which is a sign of improving the apoptotic pathway of malignant cells. It also displayed marked fibrosis at most of the malignant cells and the development of some regenerative nodules. Heliomycin showed moderate immunoreactivity with alpha-fetoprotein (AFP), and proliferation cell nuclear antigen (PCNA) compared to the positive control group. To the best of our knowledge, this is the first study to report the anticancer activity of heliomycin against hepatocellular carcinoma in vivo.
Identifiants
pubmed: 33416934
doi: 10.1007/s00210-020-02043-5
pii: 10.1007/s00210-020-02043-5
doi:
Substances chimiques
Anticarcinogenic Agents
0
Polycyclic Compounds
0
alpha-Fetoproteins
0
Diethylnitrosamine
3IQ78TTX1A
heliomycin
7N3A092A5X
Aspartate Aminotransferases
EC 2.6.1.1
Alanine Transaminase
EC 2.6.1.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1091-1102Références
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