Identification of protein/mRNA network involving the PSORS1 locus gene CCHCR1 and the PSORS4 locus gene HAX1.
Adaptor Proteins, Signal Transducing
/ genetics
Animals
Cells, Cultured
Gene Regulatory Networks
/ genetics
Genetic Loci
Genetic Predisposition to Disease
/ genetics
HL-60 Cells
HeLa Cells
Humans
Infant, Newborn
Intracellular Signaling Peptides and Proteins
/ genetics
Male
Polymorphism, Single Nucleotide
Protein Binding
Protein Interaction Maps
/ genetics
Psoriasis
/ genetics
RNA, Messenger
/ analysis
Rabbits
CCHCR1
HAX1
LL37
Psoriasis
RNA metabolism
Vesicular trafficking
Vimentin
Journal
Experimental cell research
ISSN: 1090-2422
Titre abrégé: Exp Cell Res
Pays: United States
ID NLM: 0373226
Informations de publication
Date de publication:
15 02 2021
15 02 2021
Historique:
received:
05
06
2020
revised:
22
12
2020
accepted:
30
12
2020
pubmed:
9
1
2021
medline:
12
6
2021
entrez:
8
1
2021
Statut:
ppublish
Résumé
CCHCR1 (Coiled-Coil alpha-Helical Rod 1), maps to chromosomal region 6p21.3, within the major psoriasis susceptibility locus PSORS1. CCHCR1 itself is a plausible psoriasis candidate gene, however its role in psoriasis pathogenesis remains unclear. We previously demonstrated that CCHCR1 protein acts as a cytoplasmic docking site for RNA polymerase II core subunit 3 (RPB3) in cycling cells, suggesting a role for CCHCR1 in vesicular trafficking between cellular compartments. Here, we report a novel interaction between CCHCR1 and the RNA binding protein HAX1. HAX1 maps to chromosomal region 1q21.3 within the PSORS4 locus and is over-expressed in psoriasis. Both CCHCR1 and HAX1 share subcellular co-localization with mitochondria, nuclei and cytoplasmic vesicles as P-bodies. By a series of ribonucleoprotein immunoprecipitation (RIP) assays, we isolated a pool of mRNAs complexed with HAX1 and/or CCHCR1 proteins. Among the mRNAs complexed with both CCHCR1 and HAX1 proteins, there are Vimentin mRNA, previously described to be bound by HAX1, and CAMP/LL37 mRNA, whose gene product is over-expressed in psoriasis.
Identifiants
pubmed: 33417922
pii: S0014-4827(21)00002-1
doi: 10.1016/j.yexcr.2021.112471
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
CCHCR1 protein, human
0
HAX1 protein, human
0
Intracellular Signaling Peptides and Proteins
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
112471Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.