Identification of Key Phospholipids That Bind and Activate Atypical PKCs.
kinase regulation
lipid signalling
lipid-protein interaction
membrane
phosphatidylinositols
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
06 Jan 2021
06 Jan 2021
Historique:
received:
30
11
2020
revised:
30
12
2020
accepted:
01
01
2021
entrez:
9
1
2021
pubmed:
10
1
2021
medline:
10
1
2021
Statut:
epublish
Résumé
PKCζ and PKCι/λ form the atypical protein kinase C subgroup, characterised by a lack of regulation by calcium and the neutral lipid diacylglycerol. To better understand the regulation of these kinases, we systematically explored their interactions with various purified phospholipids using the lipid overlay assays, followed by kinase activity assays to evaluate the lipid effects on their enzymatic activity. We observed that both PKCζ and PKCι interact with phosphatidic acid and phosphatidylserine. Conversely, PKCι is unique in binding also to phosphatidylinositol-monophosphates (e.g., phosphatidylinositol 3-phosphate, 4-phosphate, and 5-phosphate). Moreover, we observed that phosphatidylinositol 4-phosphate specifically activates PKCι, while both isoforms are responsive to phosphatidic acid and phosphatidylserine. Overall, our results suggest that atypical Protein kinase C (PKC) localisation and activity are regulated by membrane lipids distinct from those involved in conventional PKCs and unveil a specific regulation of PKCι by phosphatidylinositol-monophosphates.
Identifiants
pubmed: 33419210
pii: biomedicines9010045
doi: 10.3390/biomedicines9010045
pmc: PMC7825596
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
J Biol Chem. 2009 May 22;284(21):14469-75
pubmed: 19304661
J Cell Biol. 2020 Jul 6;219(7):
pubmed: 32580209
Cell Death Differ. 2019 May;26(5):781-793
pubmed: 30742090
Biochem J. 2002 Mar 15;362(Pt 3):665-74
pubmed: 11879194
J Neurosci Res. 1999 Feb 1;55(3):293-302
pubmed: 10348660
Mol Cell Biol. 2014 Jan;34(1):84-95
pubmed: 24164897
PLoS One. 2014 Jun 02;9(6):e97144
pubmed: 24887021
Bioessays. 2013 Jul;35(7):612-22
pubmed: 23712958
Cancer Cell. 2019 Sep 16;36(3):218-235
pubmed: 31474570
EMBO J. 1995 May 1;14(9):1961-9
pubmed: 7744003
J Cell Physiol. 2011 Apr;226(4):879-87
pubmed: 20945390
J Biol Chem. 2005 Sep 2;280(35):31109-15
pubmed: 15994303
Cell Mol Life Sci. 2013 Sep;70(17):3057-66
pubmed: 23096778
Sci Signal. 2019 Jan 01;12(562):
pubmed: 30600259
J Biol Chem. 2005 Jul 15;280(28):26415-24
pubmed: 15901738
Chem Biol. 2011 Nov 23;18(11):1463-73
pubmed: 22118680
Mol Biol Cell. 2002 Oct;13(10):3730-46
pubmed: 12388770
FEBS J. 2017 May;284(9):1267-1278
pubmed: 27973739
Cancer Res. 2005 Oct 1;65(19):8905-11
pubmed: 16204062
Biochim Biophys Acta. 2016 Mar;1861(3):249-59
pubmed: 26743850
Exp Cell Res. 2014 Nov 1;328(2):296-302
pubmed: 25128813
J Biol Chem. 2003 Oct 10;278(41):40305-16
pubmed: 12857744
Cancer Res. 2006 Jun 15;66(12):6296-303
pubmed: 16778206
Oncogene. 2008 Aug 14;27(35):4841-53
pubmed: 18427549
J Biol Chem. 2006 Nov 3;281(44):33773-88
pubmed: 16950780
Sci Rep. 2019 Oct 10;9(1):14527
pubmed: 31601855
F1000Res. 2018 Jun 25;7:
pubmed: 29983916
J Biol Chem. 2010 Nov 12;285(46):35979-87
pubmed: 20826779
J Immunol. 2001 Jan 15;166(2):1206-13
pubmed: 11145703
Biochem J. 1994 Dec 15;304 ( Pt 3):1001-8
pubmed: 7818462
Ann Surg Oncol. 2009 Jun;16(6):1578-86
pubmed: 19290490
J Lipid Res. 2004 Mar;45(3):496-506
pubmed: 14657198
Proc Natl Acad Sci U S A. 2005 Aug 30;102(35):12519-24
pubmed: 16116079
Exp Cell Res. 2013 Jun 10;319(10):1357-64
pubmed: 23535009
Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4182-7
pubmed: 20160093
Nat Cell Biol. 2006 Nov;8(11):1235-45
pubmed: 17060907
Biochim Biophys Acta. 2006 Jul;1761(7):633-54
pubmed: 16809062
J Biol Chem. 2006 Sep 22;281(38):28450-9
pubmed: 16861740
J Mol Biol. 2016 Apr 10;428(7):1455-64
pubmed: 26992354
Mol Biol Cell. 2007 Dec;18(12):4859-71
pubmed: 17898083
J Biol Chem. 2000 Nov 10;275(45):35617-23
pubmed: 10962008
Cell Death Differ. 2014 Sep;21(9):1469-81
pubmed: 24786829
J Biol Chem. 1993 Jan 5;268(1):13-6
pubmed: 8380153
EMBO Rep. 2014 Feb;15(2):191-8
pubmed: 24375676
J Biol Chem. 2014 Sep 5;289(36):25021-30
pubmed: 25035426
Int J Mol Sci. 2017 Mar 15;18(3):
pubmed: 28294977
EMBO J. 1996 Feb 15;15(4):788-98
pubmed: 8631300
Hum Pathol. 2008 Jun;39(6):824-31
pubmed: 18538170
Curr Biol. 1998 Sep 24;8(19):1069-77
pubmed: 9768361
J Biol Chem. 2007 Apr 27;282(17):12450-7
pubmed: 17308302
Int J Mol Sci. 2019 Apr 27;20(9):
pubmed: 31035587
Biochemistry. 2001 Jan 9;40(1):249-55
pubmed: 11141077
Biochem Pharmacol. 2014 Mar 1;88(1):1-11
pubmed: 24231509
J Biol Chem. 2011 Mar 11;286(10):8149-57
pubmed: 21189248
Chem Biol. 2014 Jun 19;21(6):754-65
pubmed: 24836908
Oncogene. 2009 Oct 15;28(41):3597-607
pubmed: 19617897
Hepatology. 2017 Oct;66(4):1165-1182
pubmed: 28574228
Methods Enzymol. 2017;583:327-357
pubmed: 28063497
Mol Cell Biol. 1998 May;18(5):3069-80
pubmed: 9566925
Exp Mol Pathol. 2019 Apr;107:57-67
pubmed: 30465755
Biochem J. 2016 Feb 15;473(4):509-23
pubmed: 26635352
Sci STKE. 2002 Apr 23;2002(129):pl6
pubmed: 11972359
Biochim Biophys Acta. 2005 Jul 10;1744(3):396-405
pubmed: 15979509
J Immunol. 2004 Sep 1;173(5):3250-60
pubmed: 15322187