Cell Type-Specific Decomposition of Gingival Tissue Transcriptomes.
B cells
epithelial cells
gene expression
periodontitis
reverse transcription polymerase chain reaction
validation
Journal
Journal of dental research
ISSN: 1544-0591
Titre abrégé: J Dent Res
Pays: United States
ID NLM: 0354343
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
pubmed:
10
1
2021
medline:
29
4
2021
entrez:
9
1
2021
Statut:
ppublish
Résumé
Genome-wide transcriptomic analyses in whole tissues reflect the aggregate gene expression in heterogeneous cell populations comprising resident and migratory cells, and they are unable to identify cell type-specific information. We used a computational method (population-specific expression analysis [PSEA]) to decompose gene expression in gingival tissues into cell type-specific signatures for 8 cell types (epithelial cells, fibroblasts, endothelial cells, neutrophils, monocytes/macrophages, plasma cells, T cells, and B cells). We used a gene expression data set generated using microarrays from 120 persons (310 tissue samples; 241 periodontitis affected and 69 healthy). Decomposition of the whole-tissue transcriptomes identified differentially expressed genes in each of the cell types, which mapped to biologically relevant pathways, including dysregulation of Th17 cell differentiation, AGE-RAGE signaling, and epithelial-mesenchymal transition in epithelial cells. We validated selected PSEA-predicted, differentially expressed genes in purified gingival epithelial cells and B cells from an unrelated cohort (
Identifiants
pubmed: 33419383
doi: 10.1177/0022034520979614
pmc: PMC8060774
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
549-556Subventions
Organisme : NIDCR NIH HHS
ID : R03 DE024735
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR000040
Pays : United States
Organisme : NIDCR NIH HHS
ID : R03 DE029546
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE015649
Pays : United States
Organisme : NIDCR NIH HHS
ID : R21 DE021820
Pays : United States
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