Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin.
Administration, Cutaneous
Adult
Anti-Inflammatory Agents
/ administration & dosage
Female
Gas Chromatography-Mass Spectrometry
Humans
Hydrogels
Imines
Middle Aged
Peptide Hydrolases
/ metabolism
Protease Inhibitors
/ administration & dosage
Proteomics
Pyridines
/ administration & dosage
Skin
/ chemistry
Wound Healing
/ drug effects
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
08 01 2021
08 01 2021
Historique:
received:
18
08
2020
accepted:
04
12
2020
entrez:
9
1
2021
pubmed:
10
1
2021
medline:
20
7
2021
Statut:
epublish
Résumé
Octenidine dihydrochloride (OCT) is a widely used antiseptic molecule, promoting skin wound healing accompanied with improved scar quality after surgical procedures. However, the mechanisms by which OCT is contributing to tissue regeneration are not yet completely clear. In this study, we have used a superficial wound model by tape stripping of ex vivo human skin. Protein profiles of wounded skin biopsies treated with OCT-containing hydrogel and the released secretome were analyzed using liquid chromatography-mass spectrometry (LC-MS) and enzyme-linked immunosorbent assay (ELISA), respectively. Proteomics analysis of OCT-treated skin wounds revealed significant lower levels of key players in tissue remodeling as well as reepithelization after wounding such as pro-inflammatory cytokines (IL-8, IL-6) and matrix-metalloproteinases (MMP1, MMP2, MMP3, MMP9) when compared to controls. In addition, enzymatic activity of several released MMPs into culture supernatants was significantly lower in OCT-treated samples. Our data give insights on the mode of action based on which OCT positively influences wound healing and identified anti-inflammatory and protease-inhibitory activities of OCT.
Identifiants
pubmed: 33420112
doi: 10.1038/s41598-020-79378-9
pii: 10.1038/s41598-020-79378-9
pmc: PMC7794247
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Hydrogels
0
Imines
0
Protease Inhibitors
0
Pyridines
0
Peptide Hydrolases
EC 3.4.-
octenidine
OZE0372S5A
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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