Thioredoxin Delays Photoreceptor Degeneration, Oxidative and Inflammation Alterations in Retinitis Pigmentosa.
glia
glutathione
hepatic growth factor
retina
thioredoxin
vascular endothelial growth factor
Journal
Frontiers in pharmacology
ISSN: 1663-9812
Titre abrégé: Front Pharmacol
Pays: Switzerland
ID NLM: 101548923
Informations de publication
Date de publication:
2020
2020
Historique:
received:
02
08
2020
accepted:
03
11
2020
entrez:
11
1
2021
pubmed:
12
1
2021
medline:
12
1
2021
Statut:
epublish
Résumé
Retinitis pigmentosa (RP) is an inherited ocular disorder with no effective treatment. RP onset and progression trigger a cascade of retinal disorders that lead to the death of photoreceptors. After photoreceptors death, neuronal, glial and vascular remodeling can be observed in the retina. The purpose of this study was to study if thioredoxin (TRX) administration is able to decrease photoreceptor death in an animal model of RP (rd1 mouse), but also if it is able to modulate the retinal oxidative stress, glial and vascular changes that can be observed as the disease progresses. Wild type and rd1 mice received several doses of TRX. After treatment, animals were euthanized at postnatals days 11, 17, or 28. Glutathione (GSH) and other thiol compounds were determined by high performance liquid chromatography (HPLC). Glial fibrilary acidic protein (GFAP) and anti-ionized calcium binding adaptor molecule 1 (Iba1) were studied by immunohistochemistry. Vascular endothelial growth factor (VEGF) and hepatic growth factor (HGF) expression were determined by western blot. TRX administration significantly diminished cell death in rd1 mouse retinas and increased GSH retinal concentrations at postnatal day 11 (PN11). TRX was also able to reverse glial alterations at PN11 and PN17. No alterations were observed in retinal VEGF and HGF expression in rd1 mice. In conclusion, TRX treatment decreases photoreceptor death in the first stages of RP and this protective effect may be due in part to the GSH system activation and to a partially decrease in inflammation.
Identifiants
pubmed: 33424600
doi: 10.3389/fphar.2020.590572
pii: 590572
pmc: PMC7785808
doi:
Types de publication
Journal Article
Langues
eng
Pagination
590572Informations de copyright
Copyright © 2020 Gimeno-Hernández, Cantó, Fernández-Carbonell, Olivar, Hernández-Rabaza, Almansa and Miranda.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Clin Genet. 2013 Aug;84(2):132-41
pubmed: 23701314
Sci Transl Med. 2010 Apr 7;2(26):26ps16
pubmed: 20375363
Med Res Rev. 2004 Jan;24(1):40-89
pubmed: 14595672
PLoS One. 2011;6(6):e21193
pubmed: 21738619
Neuro Endocrinol Lett. 2019 Mar;39(8):561-566
pubmed: 30927761
Nat Genet. 2004 Jul;36(7):755-9
pubmed: 15220920
Ophthalmology. 2013 Jan;120(1):e5-12
pubmed: 22986110
Nature. 1997 Nov 27;390(6658):404-7
pubmed: 9389480
Free Radic Biol Med. 2001 Jun 1;30(11):1191-212
pubmed: 11368918
Invest Ophthalmol Vis Sci. 2008 Aug;49(8):3499-502
pubmed: 18326689
Exp Brain Res. 1998 Aug;121(4):419-24
pubmed: 9746148
Invest Ophthalmol Vis Sci. 2002 Jan;43(1):267-73
pubmed: 11773041
Neuropharmacology. 2014 Feb;77:453-64
pubmed: 24212059
J Clin Endocrinol Metab. 1999 Feb;84(2):659-62
pubmed: 10022434
Antioxid Redox Signal. 2004 Feb;6(1):81-7
pubmed: 14713338
Antioxid Redox Signal. 2000 Winter;2(4):811-20
pubmed: 11213485
Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15143-8
pubmed: 11742067
J Korean Med Sci. 2014 Jan;29(1):117-21
pubmed: 24431915
Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):10242-7
pubmed: 10963684
Exp Eye Res. 2005 Aug;81(2):123-37
pubmed: 15916760
Neuroscience. 2007 Mar 30;145(3):1120-9
pubmed: 17293057
Cell. 2010 Mar 19;140(6):918-34
pubmed: 20303880
Exp Eye Res. 1979 Jan;28(1):63-9
pubmed: 376324
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Br J Ophthalmol. 1995 Feb;79(2):186-90
pubmed: 7696244
Prog Retin Eye Res. 2008 Jul;27(4):331-71
pubmed: 18653375
Adv Drug Deliv Rev. 2009 Apr 28;61(4):303-9
pubmed: 19385090
J Cell Biol. 1992 Nov;119(3):629-41
pubmed: 1383237
Eur J Ophthalmol. 2003 Apr;13 Suppl 3:S44-56
pubmed: 12749677
Eur J Clin Invest. 1999 Mar;29(3):238-45
pubmed: 10202381
J Cell Physiol. 2005 Jun;203(3):457-64
pubmed: 15744744
Invest Ophthalmol Vis Sci. 1980 Jan;19(1):94-6
pubmed: 6765938
Med Hypotheses. 2012 Apr;78(4):423-7
pubmed: 22284635
Tohoku J Exp Med. 2000 Aug;191(4):221-32
pubmed: 11038014
Annu Rev Biophys Biomol Struct. 2001;30:421-55
pubmed: 11441809
Semin Cancer Biol. 2006 Dec;16(6):420-6
pubmed: 17092741
Proc Natl Acad Sci U S A. 1924 Jul;10(7):329-33
pubmed: 16576828
Neuroscience. 2020 Jan 15;425:235-250
pubmed: 31785355
BMC Mol Biol. 2007 Aug 31;8:74
pubmed: 17764561
Antioxid Redox Signal. 2013 Nov 1;19(13):1539-605
pubmed: 23397885
Sci Transl Med. 2016 Dec 7;8(368):368rv6
pubmed: 27928030
J Neurosci. 1999 Jul 15;19(14):5731-40
pubmed: 10407014
Prog Retin Eye Res. 2016 Mar;51:1-40
pubmed: 26113209
Diabetes Res Clin Pract. 2006 Jan;71(1):36-44
pubmed: 16029908
Arch Ophthalmol. 2010 Apr;128(4):403-11
pubmed: 20385935
Pharmacol Res. 2014 Sep;87:8-17
pubmed: 24928737
Pharmacol Res. 2015 Sep;99:276-88
pubmed: 26158501
Neurosci Lett. 1995 Jun 23;193(1):25-8
pubmed: 7566658
Invest Ophthalmol Vis Sci. 1978 Jun;17(6):489-98
pubmed: 659071
Am J Hum Genet. 1998 Mar;62(3):503-8
pubmed: 9497269
J Neurochem. 2006 Feb;96(3):802-14
pubmed: 16405498
J Cell Physiol. 2019 Apr;234(4):4256-4266
pubmed: 30191983
Arch Ophthalmol. 1996 Jan;114(1):79-83
pubmed: 8540855
Am J Hum Genet. 1980 Mar;32(2):223-35
pubmed: 7386458
Nature. 1990 Oct 18;347(6294):677-80
pubmed: 1977087
Trends Immunol. 2008 Aug;29(8):357-65
pubmed: 18599350
Invest Ophthalmol Vis Sci. 2007 Jun;48(6):2760-8
pubmed: 17525210
Exp Eye Res. 2016 Sep;150:149-65
pubmed: 27020758
Invest Ophthalmol Vis Sci. 2010 Jun;51(6):3157-61
pubmed: 20053975
Open Ophthalmol J. 2013;7:4-10
pubmed: 23459241
J Cell Physiol. 2011 Jul;226(7):1843-9
pubmed: 21506115
Br J Ophthalmol. 1981 Dec;65(12):855-8
pubmed: 7317333
J Comp Neurol. 1986 Dec 22;254(4):543-53
pubmed: 3805362
Anal Biochem. 1980 Jul 15;106(1):55-62
pubmed: 7416469
Vision Res. 1980;20(12):1157-61
pubmed: 6267810
Am J Ophthalmol. 1996 Oct;122(4):502-8
pubmed: 8862046
Nat Rev Genet. 2010 Apr;11(4):273-84
pubmed: 20212494
Biochim Biophys Acta. 2012 Oct;1823(10):1767-77
pubmed: 22732297
Invest Ophthalmol Vis Sci. 2011 Jul 01;52(8):4998-5008
pubmed: 21508111
J Steroid Biochem Mol Biol. 2019 May;189:291-301
pubmed: 30654106
J Biol Chem. 1951 Nov;193(1):265-75
pubmed: 14907713
FASEB Bioadv. 2020 Feb 18;2(4):219-233
pubmed: 32259049
Brain Res. 2015 Jul 30;1615:89-97
pubmed: 25935696