Appropriate Activity Assays Are Crucial for the Specific Determination of Proline Dehydrogenase and Pyrroline-5-Carboxylate Reductase Activities.
electron acceptor
enzyme activity assay
mitochondria
proline dehydrogenase
protein extraction
pyrroline-5-carboxylate reductase
Journal
Frontiers in plant science
ISSN: 1664-462X
Titre abrégé: Front Plant Sci
Pays: Switzerland
ID NLM: 101568200
Informations de publication
Date de publication:
2020
2020
Historique:
received:
04
09
2020
accepted:
03
12
2020
entrez:
11
1
2021
pubmed:
12
1
2021
medline:
12
1
2021
Statut:
epublish
Résumé
Accumulation of proline is a widespread plant response to a broad range of environmental stress conditions including salt and osmotic stress. Proline accumulation is achieved mainly by upregulation of proline biosynthesis in the cytosol and by inhibition of proline degradation in mitochondria. Changes in gene expression or activity levels of the two enzymes catalyzing the first reactions in these two pathways, namely pyrroline-5-carboxylate (P5C) synthetase and proline dehydrogenase (ProDH), are often used to assess the stress response of plants. The difficulty to isolate ProDH in active form has led several researchers to erroneously report proline-dependent NAD
Identifiants
pubmed: 33424902
doi: 10.3389/fpls.2020.602939
pmc: PMC7785524
doi:
Types de publication
Journal Article
Langues
eng
Pagination
602939Informations de copyright
Copyright © 2020 Lebreton, Cabassa-Hourton, Savouré, Funck and Forlani.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):3389-94
pubmed: 24550478
Plant Physiol. 1992 Aug;99(4):1642-9
pubmed: 16669085
Plant Mol Biol. 2018 Sep;98(1-2):121-135
pubmed: 30143990
Plant Physiol. 1982 Aug;70(2):567-72
pubmed: 16662535
Biochem J. 2016 Sep 1;473(17):2623-34
pubmed: 27303048
New Phytol. 2014 May;202(3):911-9
pubmed: 24467670
Metabolites. 2020 Apr 13;10(4):
pubmed: 32295054
Front Plant Sci. 2015 Jul 28;6:565
pubmed: 26284087
Plant Physiol Biochem. 2020 Sep;154:268-276
pubmed: 32574985
J Mol Biol. 2005 Nov 18;354(1):91-106
pubmed: 16233902
Plant Physiol. 1997 Apr;113(4):1413-1418
pubmed: 12223682
Plant Physiol. 1974 Mar;53(3):440-4
pubmed: 16658720
New Phytol. 2015 Oct;208(2):354-69
pubmed: 26037253
Arch Biochem Biophys. 2010 Jun 15;498(2):136-42
pubmed: 20450881
Plant Physiol. 1981 Apr;67(4):780-4
pubmed: 16661754
Front Plant Sci. 2020 Jul 23;11:1127
pubmed: 32793273
Plant Physiol. 1986 Jan;80(1):142-4
pubmed: 16664571
J Mol Biol. 2006 Jun 23;359(5):1364-77
pubmed: 16730026
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
Front Plant Sci. 2019 Dec 10;10:1584
pubmed: 31921239
Antioxid Redox Signal. 2019 Feb 1;30(4):650-673
pubmed: 28990412
Biochemistry. 1995 Sep 12;34(36):11515-20
pubmed: 7547881
Plant Cell Physiol. 2001 Jul;42(7):742-50
pubmed: 11479381
Mol Gen Genet. 1988 Nov;214(3):379-88
pubmed: 2464125
Trends Plant Sci. 2010 Feb;15(2):89-97
pubmed: 20036181
BMC Plant Biol. 2012 Oct 13;12:191
pubmed: 23062072
Plant J. 2001 Aug;27(4):345-56
pubmed: 11532180
Plant Physiol. 1979 Mar;63(3):531-5
pubmed: 16660761
Front Biosci (Landmark Ed). 2012 Jan 01;17:607-20
pubmed: 22201764
BMC Plant Biol. 2010 Apr 19;10:70
pubmed: 20403182
Plant Physiol. 1991 Mar;95(3):787-91
pubmed: 16668054
Protein Expr Purif. 2012 Apr;82(2):345-51
pubmed: 22333530
J Agric Food Chem. 2007 May 30;55(11):4340-7
pubmed: 17474756
Plant Physiol Biochem. 2017 Jun;115:126-140
pubmed: 28364709
Front Plant Sci. 2015 Aug 05;6:591
pubmed: 26300893
Nucleic Acids Res. 2012 Jan;40(Database issue):D1211-5
pubmed: 22080561
J Exp Bot. 2019 Nov 18;70(21):6203-6214
pubmed: 31504781
Plant Physiol. 1989 Oct;91(2):581-6
pubmed: 16667072
FEBS J. 2014 Jun;281(12):2794-804
pubmed: 24751239
Front Plant Sci. 2020 Oct 22;11:582026
pubmed: 33193529
J Biol Chem. 1951 Nov;193(1):265-75
pubmed: 14907713