Effect of Age and Lipoperoxidation in Rat and Human Adipose Tissue-Derived Stem Cells.
AMP-Activated Protein Kinase Kinases
Adipose Tissue
/ cytology
Adult
Age Factors
Animals
Apoptosis
Cell Differentiation
Cell Proliferation
Female
HeLa Cells
Humans
Lipid Peroxidation
Male
Mesenchymal Stem Cells
/ cytology
Middle Aged
Nanog Homeobox Protein
/ metabolism
Oxidative Stress
Peptide Elongation Factor 2
/ metabolism
Protein Kinases
/ metabolism
Rats
Rats, Wistar
Regenerative Medicine
SOXB1 Transcription Factors
/ metabolism
Sirtuin 1
/ metabolism
Stem Cells
/ cytology
Time Factors
Journal
Oxidative medicine and cellular longevity
ISSN: 1942-0994
Titre abrégé: Oxid Med Cell Longev
Pays: United States
ID NLM: 101479826
Informations de publication
Date de publication:
2020
2020
Historique:
received:
11
06
2020
revised:
08
11
2020
accepted:
19
11
2020
entrez:
11
1
2021
pubmed:
12
1
2021
medline:
14
9
2021
Statut:
epublish
Résumé
A wide range of clinical applications in regenerative medicine were opened decades ago with the discovery of adult stem cells. Highly promising adult stem cells are mesenchymal stem/stromal cells derived from adipose tissue (ADSCs), primarily because of their abundance and accessibility. These cells have multipotent properties and have been used extensively to carry out autologous transplants. However, the biology of these cells is not entirely understood. Among other factors, the regeneration capacity of these cells will depend on both their capacity of proliferation/differentiation and the robustness of the biochemical pathways that allow them to survive under adverse conditions like those found in damaged tissues. The transcription factors, such as Nanog and Sox2, have been described as playing an important role in stem cell proliferation and differentiation. Also, the so-called longevity pathways, in which AMPK and SIRT1 proteins play a crucial role, are essential for cell homeostasis under stressful situations. These pathways act by inhibiting the translation through downregulation of elongation factor-2 (eEF2). In order to deepen knowledge of mesenchymal stem cell biology and which factors are determinant in the final therapeutic output, we evaluate in the present study the levels of all of these proteins in the ADSCs from humans and rats and how these levels are affected by aging and the oxidative environment. Due to the effect of aging and oxidative stress, our results suggest that before performing a cell therapy with ADSCs, several aspects reported in this study such as oxidative stress status and proliferation and differentiation capacity should be assessed on these cells. This would allow us to know the robustness of the transplanted cells and to predict the therapeutic result, especially in elder patients, where probably ADSCs do not carry out their biological functions in an optimal way.
Identifiants
pubmed: 33425211
doi: 10.1155/2020/6473279
pmc: PMC7775166
doi:
Substances chimiques
NANOG protein, human
0
Nanog Homeobox Protein
0
Nanog protein, rat
0
Peptide Elongation Factor 2
0
SOX2 protein, human
0
SOXB1 Transcription Factors
0
Sox2 protein, rat
0
Protein Kinases
EC 2.7.-
AMP-Activated Protein Kinase Kinases
EC 2.7.11.3
SIRT1 protein, human
EC 3.5.1.-
Sirt1 protein, rat
EC 3.5.1.-
Sirtuin 1
EC 3.5.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6473279Informations de copyright
Copyright © 2020 Mario F. Muñoz et al.
Déclaration de conflit d'intérêts
The authors declare that they have no conflicts of interest.
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