CYP450 core involvement in multiple resistance strains of Aedes aegypti from French Guiana highlighted by proteomics, molecular and biochemical studies.
Aedes
/ drug effects
Animals
Cytochrome P-450 Enzyme System
/ genetics
Esterases
/ metabolism
Female
French Guiana
Gene Knockdown Techniques
Genotype
Inactivation, Metabolic
/ genetics
Insect Proteins
/ antagonists & inhibitors
Insecticide Resistance
/ genetics
Insecticides
/ pharmacology
Intestinal Mucosa
/ metabolism
Nitriles
/ pharmacology
Oligonucleotides
/ metabolism
Polymorphism, Single Nucleotide
Proteome
/ analysis
Proteomics
Pyrethrins
/ pharmacology
Voltage-Gated Sodium Channels
/ chemistry
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
15
07
2020
accepted:
02
12
2020
entrez:
11
1
2021
pubmed:
12
1
2021
medline:
24
4
2021
Statut:
epublish
Résumé
Insecticide resistance is a worldwide threat for vector control around the world, and Aedes aegypti, the main vector of several arboviruses, is a particular concern. To better understand the mechanisms of resistance, four isofemale strains originally from French Guiana were isolated and analysed using combined approaches. The activity of detoxification enzymes involved in insecticide resistance was assayed, and mutations located at positions 1016 and 1534 of the sodium voltage-gated channel gene, which have been associated with pyrethroid resistance in Aedes aegypti populations in Latin America, were monitored. Resistance to other insecticide families (organophosphates and carbamates) was evaluated. A large-scale proteomic analysis was performed to identify proteins involved in insecticide resistance. Our results revealed a metabolic resistance and resistance associated with a mutation of the sodium voltage-gated channel gene at position 1016. Metabolic resistance was mediated through an increase of esterase activity in most strains but also through the shifts in the abundance of several cytochrome P450 (CYP450s). Overall, resistance to deltamethrin was linked in the isofemale strains to resistance to other class of insecticides, suggesting that cross- and multiple resistance occur through selection of mechanisms of metabolic resistance. These results give some insights into resistance to deltamethrin and into multiple resistance phenomena in populations of Ae. aegypti.
Identifiants
pubmed: 33428654
doi: 10.1371/journal.pone.0243992
pii: PONE-D-20-21931
pmc: PMC7799788
doi:
Substances chimiques
Insect Proteins
0
Insecticides
0
Nitriles
0
Oligonucleotides
0
Proteome
0
Pyrethrins
0
Voltage-Gated Sodium Channels
0
decamethrin
2JTS8R821G
Cytochrome P-450 Enzyme System
9035-51-2
Esterases
EC 3.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0243992Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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