Volumetric Absorptive Microsampling of Blood for Untargeted Lipidomics.
DBS
VAMS
lipidomics
microsampling
whole blood
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
07 Jan 2021
07 Jan 2021
Historique:
received:
12
12
2020
revised:
28
12
2020
accepted:
04
01
2021
entrez:
12
1
2021
pubmed:
13
1
2021
medline:
9
9
2021
Statut:
epublish
Résumé
In the present, proof-of-concept paper, we explore the potential of one common solid support for blood microsampling (dried blood spot, DBS) and a device (volumetric absorptive microsampling, VAMS) developed for the untargeted lipidomic profiling of human whole blood, performed by high-resolution LC-MS/MS. Dried blood microsamples obtained by means of DBS and VAMS were extracted with different solvent compositions and compared with fluid blood to evaluate their efficiency in profiling the lipid chemical space in the most broad way. Although more effort is needed to better characterize this approach, our results indicate that VAMS is a viable option for untargeted studies and its use will bring all the corresponding known advantages in the field of lipidomics, such as haematocrit independence.
Identifiants
pubmed: 33430231
pii: molecules26020262
doi: 10.3390/molecules26020262
pmc: PMC7825730
pii:
doi:
Substances chimiques
Lipids
0
Solvents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
J Lipid Res. 2018 Oct;59(10):2001-2017
pubmed: 30115755
Biomed Chromatogr. 2019 Jun;33(6):e4491
pubmed: 30663096
Anal Chem. 2018 Jun 5;90(11):6494-6501
pubmed: 29708737
Lipids Health Dis. 2019 Feb 2;18(1):38
pubmed: 30711004
Curr Protoc Bioinformatics. 2016 Mar 24;53:14.13.1-14.13.18
pubmed: 27010336
Nutr Metab (Lond). 2017 Mar 20;14:28
pubmed: 28344632
Mol Cell Proteomics. 2017 Jul;16(7):1286-1296
pubmed: 28501802
Anal Bioanal Chem. 2017 Oct;409(26):6263-6276
pubmed: 28815270
Metabolomics. 2014;10(5):1018-1025
pubmed: 25177234
J Pharm Biomed Anal. 2016 May 10;123:186-94
pubmed: 26905673
Bioinformatics. 2018 Dec 15;34(24):4313-4314
pubmed: 29955821
Anal Bioanal Chem. 2018 Mar;410(9):2331-2341
pubmed: 29362853
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Feb 15;1044-1045:77-86
pubmed: 28088044
Anal Bioanal Chem. 2015 Jul;407(17):4879-92
pubmed: 25736245
J Lipid Res. 2017 Dec;58(12):2275-2288
pubmed: 28986437
Sci Rep. 2017 Jul 21;7(1):6149
pubmed: 28733637
Anal Chim Acta. 2019 Jan 10;1046:32-47
pubmed: 30482302
Anal Chim Acta. 2018 Sep 26;1025:141-153
pubmed: 29801603
Biochem Biophys Res Commun. 2018 Oct 7;504(3):569-575
pubmed: 29596837
Nat Rev Drug Discov. 2005 Jul;4(7):594-610
pubmed: 16052242
J Chromatogr A. 2017 Dec 29;1530:90-103
pubmed: 29146423
Anal Chem. 2014 Aug 19;86(16):8489-95
pubmed: 25058158
Clin Chem Lab Med. 2017 Mar 1;55(3):462-469
pubmed: 27732552
J Inherit Metab Dis. 2018 May;41(3):489-498
pubmed: 29209936
Curr Protoc Bioinformatics. 2016 Sep 07;55:14.10.1-14.10.91
pubmed: 27603023
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W606-12
pubmed: 17584797
Anal Chem. 2016 Oct 4;88(19):9391-9396
pubmed: 27575696
Talanta. 2019 May 15;197:49-58
pubmed: 30771966
J Sep Sci. 2018 Jan;41(1):351-372
pubmed: 28859259
Anal Bioanal Chem. 2015 Jul;407(17):5227-33
pubmed: 25963649
Clin Transl Med. 2018 Jul 30;7(1):21
pubmed: 30058036
Semin Nephrol. 2018 Mar;38(2):127-141
pubmed: 29602396
J Pharm Biomed Anal. 2018 Apr 15;152:204-214
pubmed: 29414014
Rapid Commun Mass Spectrom. 2017 Mar 15;31(5):447-456
pubmed: 27958645
Anal Chem. 2017 Jun 6;89(11):6257-6264
pubmed: 28471643
Trends Analyt Chem. 2014 Oct 1;61:192-206
pubmed: 25309011
Anal Biochem. 2013 Feb 15;433(2):181-8
pubmed: 23103340
Anal Chem. 2018 Oct 16;90(20):12230-12238
pubmed: 30204416
Eur J Drug Metab Pharmacokinet. 2019 Feb;44(1):91-102
pubmed: 29943105