Cooperative function of synaptophysin and synapsin in the generation of synaptic vesicle-like clusters in non-neuronal cells.
Animals
COS Cells
Calcium-Calmodulin-Dependent Protein Kinase Type 2
/ metabolism
Cells, Cultured
Chlorocebus aethiops
Cytosol
/ metabolism
Endocytosis
Fibroblasts
/ metabolism
Humans
Mice
Neurons
/ metabolism
Static Electricity
Synapsins
/ metabolism
Synaptic Vesicles
/ metabolism
Synaptophysin
/ metabolism
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
11 01 2021
11 01 2021
Historique:
received:
01
06
2020
accepted:
02
12
2020
entrez:
12
1
2021
pubmed:
13
1
2021
medline:
22
1
2021
Statut:
epublish
Résumé
Clusters of tightly packed synaptic vesicles (SVs) are a defining feature of nerve terminals. While SVs are mobile within the clusters, the clusters have no boundaries consistent with a liquid phase. We previously found that purified synapsin, a peripheral SV protein, can assemble into liquid condensates and trap liposomes into them. How this finding relates to the physiological formation of SV clusters in living cells remains unclear. Here, we report that synapsin alone, when expressed in fibroblasts, has a diffuse cytosolic distribution. However, when expressed together with synaptophysin, an integral SV membrane protein previously shown to be localized on small synaptic-like microvesicles when expressed in non-neuronal cells, is sufficient to organize such vesicles in clusters highly reminiscent of SV clusters and with liquid-like properties. This minimal reconstitution system can be a powerful model to gain mechanistic insight into the assembly of structures which are of fundamental importance in synaptic transmission.
Identifiants
pubmed: 33431828
doi: 10.1038/s41467-020-20462-z
pii: 10.1038/s41467-020-20462-z
pmc: PMC7801664
doi:
Substances chimiques
Synapsins
0
Synaptophysin
0
Calcium-Calmodulin-Dependent Protein Kinase Type 2
EC 2.7.11.17
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
263Subventions
Organisme : NIDA NIH HHS
ID : P30 DA018343
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK045735
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS036251
Pays : United States
Organisme : NINDS NIH HHS
ID : R37 NS036251
Pays : United States
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