The clonal structure and dynamics of the human T cell response to an organic chemical hapten.
Bayesian network
T cell receptor
cdr3 motif
contact dermatitis
human
immunology
inflammation
patch test
repertoire
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
12 01 2021
12 01 2021
Historique:
received:
27
12
2019
accepted:
12
01
2021
pubmed:
13
1
2021
medline:
4
2
2022
entrez:
12
1
2021
Statut:
epublish
Résumé
Diphenylcyclopropenone (DPC) is an organic chemical hapten which induces allergic contact dermatitis and is used in the treatment of warts, melanoma, and alopecia areata. This therapeutic setting therefore provided an opportunity to study T cell receptor (TCR) repertoire changes in response to hapten sensitization in humans. Repeated exposure to DPC induced highly dynamic transient expansions of a polyclonal diverse T cell population. The number of TCRs expanded early after sensitization varies between individuals and predicts the magnitude of the allergic reaction. The expanded TCRs show preferential TCR V and J gene usage and consist of clusters of TCRs with similar sequences, two characteristic features of antigen-driven responses. The expanded TCRs share subtle sequence motifs that can be captured using a dynamic Bayesian network. These observations suggest the response to DPC is mediated by a polyclonal population of T cells recognizing a small number of dominant antigens.
Identifiants
pubmed: 33432924
doi: 10.7554/eLife.54747
pii: 54747
pmc: PMC7880692
doi:
pii:
Substances chimiques
Allergens
0
Cyclopropanes
0
Haptens
0
Receptors, Antigen, T-Cell
0
diphenylcyclopropenone
I7G14NW5EC
Banques de données
SRA
['PRJNA592875']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2021, Ronel et al.
Déclaration de conflit d'intérêts
TR, MH, KW, TO, HS, RD, BC No competing interests declared, GM GM is an employee of Unilever PLC. Apart from GM's contribution (see author contributions), the funder was not involved in the study design, collection, analysis, and interpretation of data, the writing of this article or the decision to submit it for publication.
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