Digestion and Transport across the Intestinal Epithelium Affects the Allergenicity of Ara h 1 and 3 but Not of Ara h 2 and 6.
2S Albumins, Plant
/ pharmacokinetics
Adult
Animals
Antigens, Plant
/ toxicity
Basophils
/ drug effects
Biological Transport
Digestion
/ drug effects
Female
Humans
Intestinal Mucosa
/ drug effects
Lactoglobulins
/ pharmacokinetics
Male
Mast Cells
/ drug effects
Membrane Proteins
/ pharmacokinetics
Middle Aged
Plant Proteins
/ pharmacokinetics
Seed Storage Proteins
/ pharmacokinetics
Swine
allergenicity prediction
basophil and mast cell activation
food allergy
intestinal protein transport
protein digestion
Journal
Molecular nutrition & food research
ISSN: 1613-4133
Titre abrégé: Mol Nutr Food Res
Pays: Germany
ID NLM: 101231818
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
revised:
04
12
2020
received:
20
07
2020
pubmed:
13
1
2021
medline:
10
11
2021
entrez:
12
1
2021
Statut:
ppublish
Résumé
No accepted and validated methods are currently available which can accurately predict protein allergenicity. In this study, the role of digestion and transport on protein allergenicity is investigated. Peanut allergens (Ara h 1, 2, 3, and 6) and a milk allergen (β-lactoglobulin) are transported across pig intestinal epithelium using the InTESTine model and afterward basophil activation is measured to assess the (remaining) functional properties. Additionally, allergens are digested by pepsin prior to epithelial transport and their allergenicity is assessed in a human mast cell activation assay. Remarkably, transported Ara h 1 and 3 are not able to activate basophils, in contrast to Ara h 2 and 6. Digestion prior to transport results in a significant increase in mast cell activation of Ara h 1 and 3 dependent on the length of digestion time. Activation of mast cells by Ara h 2 and 6 is unaffected by digestion prior to transport. Digestion and transport influences the allergenicity of Ara h 1 and 3, but not of Ara h 2 and 6. The influence of digestion and transport on protein allergenicity may explain why current in vitro assays are not predictive for allergenicity.
Identifiants
pubmed: 33434390
doi: 10.1002/mnfr.202000712
pmc: PMC8047886
doi:
Substances chimiques
2S Albumins, Plant
0
Antigens, Plant
0
Ara h 1 protein, Arachis hypogaea
0
Ara h 2 allergen, Arachis hypogaea
0
Ara h 6 allergen, Arachis hypogaea
0
Lactoglobulins
0
Membrane Proteins
0
Plant Proteins
0
Seed Storage Proteins
0
allergen Ara h3
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000712Informations de copyright
© 2021 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH GmbH.
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