Functionalized terahertz plasmonic metasensors: Femtomolar-level detection of SARS-CoV-2 spike proteins.
Antibodies, Immobilized
/ chemistry
Biosensing Techniques
/ methods
COVID-19
/ diagnosis
COVID-19 Serological Testing
/ methods
Gold
/ chemistry
Humans
Immunoassay
/ methods
Limit of Detection
Metal Nanoparticles
/ chemistry
SARS-CoV-2
/ isolation & purification
Spike Glycoprotein, Coronavirus
/ analysis
COVID-19 pandemic
Femtomole-level detection
SARS-CoV-2 spike protein
Terahertz plasmonic biosensors
Toroidal metasurfaces
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
01 Apr 2021
01 Apr 2021
Historique:
received:
22
06
2020
revised:
22
10
2020
accepted:
04
01
2021
pubmed:
13
1
2021
medline:
17
2
2021
entrez:
12
1
2021
Statut:
ppublish
Résumé
Effective and efficient management of human betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 virus infection i.e., COVID-19 pandemic, required sensitive and selective sensors with short sample-to-result durations for performing desired diagnostics. In this direction, one appropriate alternative approach to detect SARS-CoV-2 virus protein at low level i.e., femtomolar (fM) is exploring plasmonic metasensor technology for COVID-19 diagnostics, which offers exquisite opportunities in advanced healthcare programs, and modern clinical diagnostics. The intrinsic merits of plasmonic metasensors stem from their capability to squeeze electromagnetic fields, simultaneously in frequency, time, and space. However, the detection of low-molecular weight biomolecules at low densities is a typical drawback of conventional metasensors that has recently been addressed using toroidal metasurface technology. This research is focused on the fabrication of a miniaturized plasmonic immunosensor based on toroidal electrodynamics concept that can sustain robustly confined plasmonic modes with ultranarrow lineshapes in the terahertz (THz) frequencies. By exciting toroidal dipole mode using our quasi-infinite metasurface and a judiciously optimized protocol based on functionalized gold nanoparticles (AuNPs) conjugated with the specific monoclonal antibody specific to spike protein (S1) of SARS-CoV-2 virus onto the metasurface, the resonance shifts for diverse concentrations of the spike protein are monitored. Possessing molecular weight around ~76 kDa allowed to detect the presence of SARS-CoV-2 virus protein with significantly low as limit of detection (LoD) was achieved as ~4.2 fM. We envisage that outcomes of this research will pave the way toward the use of toroidal metasensors as practical technologies for rapid and precise screening of SARS-CoV-2 virus carriers, symptomatic or asymptomatic, and spike proteins in hospitals, clinics, laboratories, and site of infection.
Identifiants
pubmed: 33434777
pii: S0956-5663(21)00007-5
doi: 10.1016/j.bios.2021.112971
pmc: PMC7787065
pii:
doi:
Substances chimiques
Antibodies, Immobilized
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Gold
7440-57-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112971Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
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